铜绿假单胞菌密度感应系统自体诱导因子3OC12-HSL对人肠道上皮细胞Caco-2凋亡的影响及其机制

目的 探讨铜绿假单胞菌密度感应系统自体诱导因子3OC12-HSL对肠道上皮细胞Caco-2凋亡的影响及其深入机制.方法 3OC12-HSL干扰Caco-2细胞4 h后,采用MTT法检测Caco-2细胞干扰前后细胞活性变化;Annexin V-FTTC/PI双染法流式细胞术检测细胞凋亡率的变化;Western blot检测Caco-2细胞中凋亡相关蛋白p-p38/MAPK及NF-κB表达变化.结果 MTT结果 显示,3OC12-HSL可以抑制Caco-2细胞活性,并呈剂量依赖性(P<0.05).流式细胞术结果 表明,Caco2细胞凋亡率随3OC12-HSI干扰浓度增加而增加(P<0.05).Western blot结果 显示,3OC12-HSL处理后,细胞p-p38/MAPK及NF-κB蛋白表达水平增加.结论 3OC12-HSL可以抑制肠道上皮细胞Caco-2活性,诱导细胞发生凋亡,这种作用可能和p38/MAPK通路激活以及NF-κB的激活有关.

Quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa induces the apoptosis of Caco-2 cells

Objective To investigate the effect of quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa on the apoptosis of Caco-2 cells and its mechanism. Methods Caco-2 cells were incubated with 3OC12-HSL for 4 h and then examined by MTT method for cytoactivities. Flow cytometry was used to analyze apoptosis rate of Caco-2 cells. Expression of apoptosis associated proteins p-p38/MAPK and NF-κB were detected by Western blot. Results After exposure to 3OC12-HSL for 4 h, cytoactivities of Caco-2 cells was reduced(P<0.05) with dose-dependent pattern, and higher dose of 3OC12-HSL leaded to increasing apoptosis rate of Caco-2 cells(P<0.05). 3OC12-HSL raised expression of apoptosis associated proteins p-p38/MAPK and NF-κB detected by Western blot. Conclusion Quorum sensing autoinducer 3OC12-HSL can effect cytoactivities of Caco-2 cells and may induce its apoptosis by enhancing the expression of p-p38/MAPK and NF-κB protein.