丹参注射液对急性肺损伤模型大鼠血管生成素表达的影响

目的：研究丹参注射液对急性肺损伤模型大鼠血管生成素（Ang-1）-1、Ang-2表达的影响。方法：大鼠尾静脉注射脂多糖（5mg·kg^-1）复制急性肺损伤模型。实验分为3组,即正常（生理盐水1mL）、模型（生理盐水1mL）和丹参（10g·kg^-1）组。给药12h后处死大鼠,收集肺泡灌洗液、血液、肺组织进行肺湿重/干重（W/D）值、肺系数（LI）、肺通透系数（LPI）等的计算;HE染色观察大鼠肺组织病理改变;Westernblot法测定Ang-1、Ang-2在肺组织的表达。结果：与正常组比较,模型组大鼠肺组织W/D比值、LI、LPI显著升高（P〈0.05）,且病理切片都有明显的炎症损伤改变,Ang-1表达明显减弱,Ang-2表达明显增强。与模型组比较,丹参组大鼠肺组织W/D、LI、LPI显著降低（P〈0.05）,Ang-1表达明显增强,Ang-2表达明显减弱。结论：丹参注射液对急性肺损伤有一定保护作用,其机制可能与Ang-2、Ang-1的表达调控有关。

Influence of Danshen Injection on the Expression of Angiopoietin in Model Rats with Acute Lung Injury

OBJECTIVE： To study the effects of Danshen injection on the expression of Ang-1 and Ang-2 in rats with acute lung injury. METHODS： Acute lung injury model rats were induced by lipopolysaccharide （5 mg·kg-1）via tail vein. Model rats were divided into 3 groups, i.e. normal group（normal saline 1 mL）, model group（normal saline 1 mL） and Danshen injection group（10 g·kg-1）. Those model rats were sacrificed 12 h after medication. Bronchoalveolar lavage fluid, blood samples and lung tissue were collected, and lung wet/dry （W/D） weight ratio, lung index （LI） and lung permeability index （LPI） were calculated. The pathological changes of lung tissue in rats was observed by HE staining; the expressions of Ang-1 and Ang-2 in lung tissue were determined by Western blot assay. RESULTS： Compared with normal group, W/D weight ratio, lung index （LI） and lung permeability index （LPI） were increased significantly（P0.05）, and pathological section showed that there was obvious inflammatory injury in model group. The expression of Ang-1 decreased significantly, while that of Ang-2 increased significantly. Compared with model group, the W/D, LI and LPI of Danshen injection groups decreased significantly, and the expression of Ang-1 increased significantly while that of Ang-2 decreased significantly. CONCLUSION：Danshen injection have protective effect against acute lung injury, which is associated with the expression of Ang-2 and Ang-1.