心理应激复合睡眠剥夺大鼠血清及抗氧化能力指标的变化

目的 探讨心理应激复合睡眠剥夺大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)和谷胱甘肽-过氧化物酶(GSH-PX)含量的变化.方法 将105只雄性SD大鼠,随机分为空白对照组、心理应激单因素组、心理应激阴性对照组、睡眠剥夺48 h单因素组、睡眠剥夺阴性对照组、心理应激14 d+睡眠剥夺48 h复合因素组和复合因素阴性对照组,每组15只.采用Communication Box建立心理应激模型.采用改良后的小平台法睡眠剥夺模型进行睡眠剥夺.所有动物取血清1.0 ml测定MDA含量、SOD、GSH-PX和T-AOC活力.结果 与正常对照组相比,心理应激组大鼠血清SOD活力、GSH.PX活力增加(P<0.05),T-AOC活力有增加趋势,MDA含量有降低趋势;睡眠剥夺组SOD活力增加(P<0.05)、T-AOC活力有增加趋势,GSH-PX活力、MDA含量有降低趋势;心理应激复合睡眠剥夺组SOD活力及MDA含量增加(P<0.05),T-AOC活力有增加趋势,GSH-PX活力降低(P<0.05).与心理应激和睡眠剥夺单一因素组相比,复合因素组MDA含量和T-AOC活力高于单一因素组(P<0.05),GSH-PX活力有降低趋势,SOD活力高于心理应激组,低于睡眠剥夺组.结论 心理应激、睡眠剥夺及心理应激复合睡眠剥夺后,大鼠的抗氧化能力提高,复合因素组高于单一因素组.

Effects of psychological stress combined with sleep deprivation on levels of SOD and other substances in rats

Objective To investigate effects of psychological stress combined with sleep deprivation on levels of supemxide dismutase (SOD),glutathione-superoxide(GSH-PX),T-AOC and malondialdehyde (MDA) in rats.Methods one hundred and five male SD rats were randomly divided into 7 groups,each consisting of 15 rats:the control group,the psychological stress group,the psychological stress control group,the sleep deprivation group,the sleep deprivation controt group,the psychological stress combined with sleep deprivation group and the multi-factor control group.The psychological stress model was developed with the Communication Box.Rats were deprived of sleep with the application of a small modified platform.one point zero ml of serum was taken from all the sacrificed animals to measure SOD.GsH-PX,MDA levels and T-AOC activity.Results Compared with those of the control group,SOD and GSH-PX activity increased (P<0.05), T-AOC activity had a tendency to increase.while MDA concentration showed a tendency to decrease in the psychological stress group.SOD activity of the sleep deprivation group increased (P<0.05),the T-AOC activity had a tendency to increase,while GSH-PX activity and MDA concentration showed a tendency to decrease.SOD activity and MDA level of the psychological stress combined with sleep deprivation group increased(P<0.05),the T-AOC activity showed a tendency to increase,while GSH-PX activity diminished obviously (P<0.05).Compared with the psychological stress and sleep deprivation single-factor groups,TAOC and MDA of the multi-factor group were higher than those of the single-factor group (P<0.05).The activity of GSH-PX showed a tendency to decrease,and the activity of SOD was higher than that of the psychological stress group,but lower than that of the sleep deprivation group.Conclusions Antioxidation in rats improved,following psychological stress,sleep deprivation and psychological stress combined with sleep deprivation,with the antioxidative capacity of the multi-factor group being higher than that of the single-factor group.