基于析因设计探讨附子与人参、黄芪、干姜的交互关系

目的：考察中药附子、人参、黄芪、干姜干预阿霉素（ADR）心脏毒性损伤模型大鼠的效应机制，分析附子与人参、黄芪、干姜的交互关系。方法：将72 只大鼠随机分为9 组，每组8 只。除正常组外，各组大鼠按2.5 mg·kg-1 体重剂量腹腔注射阿霉素，每周1 次，连续4 周，累计10 mg·kg-1。附子、干姜组按1.75 g·kg-1，人参、黄芪组按0.875 g·kg-1，参附、芪附组按2.625 g·kg-1，姜附组按3.5 g·kg-1 体重剂量灌胃水煎液，每日1 次，连续4 周。测定超氧化物歧化酶（SOD），肌钙蛋白（CTn）、细胞色素C（CytC）,心肌线粒体Bax、Bcl2、Caspase-3、Caspase-9 等指标，关联密切指标计算综合评分，对各项指标及综合指标进行药物组间单因素方差分析及药物间交互作用分析的析因设计方差分析。结果：模型组与正常组比较，各项指标差异均有统计学意义（P<0.05）。附子、人参、黄芪、干姜对各项指标均有不同程度影响，附子与人参、黄芪、干姜的交互作用（除Bax 外）均有统计学意义。结论：中药附子、人参、黄芪、干姜对ADR 模型大鼠心脏均具有一定的保护作用，附子与人参、黄芪、干姜配伍均能提升单一用药的效果。

Exploration of Interaction between Aconite and Ginseng, Astragalus,Dried Ginger Based on Factorial Design

This study was aimed to reveal the effector mechanisms of Chinese medicine aconite, ginseng, astragalus and dried ginger on the intervention of adriamycin (ADR) cardiotoxicity model rats. The analysis was made on the interactive relationship between aconite and ginseng, astragalus as well as dried ginger. A total of 72 rats were randomly divided into nine groups. There were eight rats in each group. Except the normal group, rats in each group were intraperitoneally injected with 2.5 mg·kg-1 of ADR according to their body weights. The injection was given once a week and continued for four weeks. The total dosage was 10 mg·kg-1. In the aconite, dried gingergroup, the intragastric administration dosage of herbal decoction was 1.75 g·kg-1. The decoction dosage in the ginseng, astragalus group was 0.875 g·kg-1. The decoction dosage in the Shenfu, Qifu group was 2.625 g·kg-1. The decoction dosage in the Jiangfu group was 3.5 g·kg-1. The intragastric administration was given once a day and continued for four weeks. Indexes such as superoxide dismutase (SOD), cardiac troponins (cTn), cytochrome C(CytC), myocardial mitochondria of Bax, Bcl 2, caspase-3, caspase-9 were detected. The colligation score was calculated associating with the close index. One-way ANOVA was given on different indexes and colligation indicators among different drug groups and the factorial design variance analysis was given to reveal the drug interactions. The results showed that compared with the normal group there were statistical significances among different indexes in the model group (P < 0.05). Aconite, ginseng, astragalus, dried ginger had varying degrees of impact on different indicators. There were statistical significances on the interaction between aconite and ginseng, astragalus, dried ginger (except Bax). It was concluded that herbal medicine aconite, ginseng, astragalus, dried ginger had certain protective effect to the heart of ADR model rats. The combination of aconite and ginseng, astragalus,dried ginger can enhance the effect compared with a single herb.