| 骨形态发生蛋白-7诱导人肾成纤维细胞间质-上皮转化的研究 |
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| 引用本文： | 黄云剑,王梓华,赵景宏,张静波. 骨形态发生蛋白-7诱导人肾成纤维细胞间质-上皮转化的研究[J]. 第三军医大学学报, 2008, 30(13): 1239-1241 |
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| 作者姓名： | 黄云剑 王梓华 赵景宏 张静波 |
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| 作者单位： | 第三军医大学新桥医院肾内科,全军肾脏病中心,重庆市肾病研究所,重庆,400037 |
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| 摘 要： | 目的 探讨骨形态发生蛋白-7(bone morphogenic protein-7,BMP-7)能否诱导人肾成纤维细胞出现间质-上皮的转化.方法 原代培养获得人肾间质成纤维细胞,传至第3代用于本实验.培养液中添加1 000 ng/L BMP-7 72 h后,观察细胞形态变化,MTT法检测细胞增殖,ELISA法测定上清Ⅰ胶原含量,Western blot分析E-cadherin和Pax2蛋白表达.结果 细胞与BMP-7孵育72 h后,出现细胞聚集类似小管细胞团块.与对照组相比,成纤维细胞增殖活性减低,Ⅰ型胶原分泌下降,上皮标志物E-cadherin和肾发生的标志分子Pax2表达增加.结论 肾成纤维细胞至少保持部分它胚胎时的印迹和可塑性,BMP-7可诱导人肾成纤维细胞呈现间质-上皮的转化.
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| 关 键 词： | 肾成纤维细胞 间质-上皮细胞的转化 |
Bone morphogenic protein-7 induces mesenchymal to epithelial transition in adult renal fibroblasts |
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| HUANG Yun-jian,WANG Zhi-hua,ZHAO Jing-hong,ZHANG Jing-bo. Bone morphogenic protein-7 induces mesenchymal to epithelial transition in adult renal fibroblasts[J]. Acta Academiae Medicinae Militaris Tertiae, 2008, 30(13): 1239-1241 |
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| Authors: | HUANG Yun-jian WANG Zhi-hua ZHAO Jing-hong ZHANG Jing-bo |
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| Affiliation: | HUANG Yun-jian,WANG Zhi-hua,ZHAO Jing-hong,ZHANG Jing-bo(Renal Disease Research Center,Department of Nephrology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China) |
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| Abstract: | Objective To explore whether bone morphogenic protein-7 can induce mesenchymal to epithelial transition in adult renal fibroblasts.Methods Human renal fibroblasts isolated from the resected kidney tissues owing to renal tumors were primarily cultured.The passage 3 renal fibroblasts were used in this experiment.The morphologic changes of the renal fibroblasts were observed by phase contrast microscopy after being subjected to DMEM-F12 control medium or medium containing 1 000 ng/ml rhBMP-7 for 72 h.The cell ... |
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| Keywords: | BMP-7 |
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