| 荧光定量PCR技术检测流产组织中人细小病毒B19的感染 |
| |
| 引用本文： | 武丽娜,梁建芳,郑绘霞,肖虹. 荧光定量PCR技术检测流产组织中人细小病毒B19的感染[J]. 山西医科大学学报, 2008, 39(1): 24-26 |
| |
| 作者姓名： | 武丽娜 梁建芳 郑绘霞 肖虹 |
| |
| 作者单位： | 山西医科大学第一临床医学院病理科,太原,030001 |
| |
| 摘 要： | 目的 比较荧光定量PCR(FQ-PCR)与PCR在检测自然流产组织人细小病毒B19(HPV B19)感染临床应用中的优劣.方法 收集自然流产病例41例以FQ-PCR与PCR分别进行流产组织的HPVB19 DNA检测.结果 FQ-PCR HPVB19DNA的阳性检出率为51.20%,PCRHPVB19DNA的阳性检出率为36.56%,两种方法的阳性检出率经统计学检验具有统计学差异(P＜0.05).结论 FQ-PCR的灵敏性较PCR高,能更加清楚地了解HPVB19在体内的存在状态.
|
| 关 键 词： | 自然流产 人类细小病毒B19 荧光定量PCR 聚合酶链反应 荧光定量 技术检测 流产组织 病毒 感染 human Determination quantitative fluorescent 状态 存在 统计学差异 验具 阳性检出率 结果 病例 自然流产 方法 临床应用 比较 |
| 文章编号： | 1007-6611（2008）01-0024-03 |
| 收稿时间： | 2007-10-15 |
| 修稿时间： | 2007-10-15 |
Determination of human parvovirus B19 (HPV B19) in abortion tissues by fluorescent quantitative PCR |
| |
| WU Lina,LIANG Jian-fang,ZHENG Hui-xia,XIAO Hong. Determination of human parvovirus B19 (HPV B19) in abortion tissues by fluorescent quantitative PCR[J]. Journal of Shanxi Medical University, 2008, 39(1): 24-26 |
| |
| Authors: | WU Lina LIANG Jian-fang ZHENG Hui-xia XIAO Hong |
| |
| Abstract: | Objective To compare the advantages and disadvantages of fluorescent quantitative PCR(FQ-PCR)and polymerase chain reaction(PCR)in determining human parvovirus B19(HPV B19)in abortion tissues.Methods Tested tissues from 41 cases of unexplained spontaneous abortion were studied using FQ-PCR and PCR assay for HPV B19.Results The prevalence of HPV B19 DNA was 51.20 % by FQ-PCR and 36.56% by PCR in the sera of abortion women,respectively.There was significant difference between them(P<0.05).Conclusion The results suggest that FQ-PCR is more sensitive and efficient than traditional PCR for deteting HPV B19 from abortion tissues. |
| |
| Keywords: | spontaneous abortion human parvovirus B19 fluorescent quantitative PCR polymerase chain reaction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
