The Effects of Di(2-Ethylhexyl)Phthalate Exposure and Selenium Nutrition on Sertoli Cell Vimentin Structure and Germ-Cell Apoptosis in Rat Testis |
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Authors: | Pinar Erkekoglu N Dilara Zeybek Belma Giray Esin Asan Filiz Hincal |
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Institution: | (1) Faculty of Pharmacy, Department of Toxicology, Hacettepe University, 06100 Ankara, Turkey;(2) Faculty of Medicine, Department of Histology and Embryology, Hacettepe University, 06100 Ankara, Turkey; |
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Abstract: | This study aimed to investigate the effects of di(2-ethylhexyl)phthalate (DEHP) on Sertoli-cell vimentin filaments and germ-cell
apoptosis in testes of pubertal rats at different selenium (Se) status. Se deficiency was produced in 3-weeks old Sprague-Dawley
rats by feeding them ≤0.05 Se mg/kg diet for 5 weeks, Se supplementation group was on 1 mg Se/kg diet, and DEHP was applied
at 1000 mg/kg dose by gavage during the last 10 days of the feeding period. The diet with excess Se did not cause any appreciable
alteration in vimentin staining and apoptosis of germ cells, but Se deficiency caused a mild decrease in the intensity of
vimentin immunoreactivity and enhanced germ-cell apoptosis significantly (approximately 3-fold, p <0.0033). DEHP exposure caused disruption and collapse of vimentin filaments and significantly induced apoptotic death of
germ cells (approximately 8-fold, p <0.0033). In DEHP-exposed Se-deficient animals, compared with the control, collapse of vimentin filaments was more prominent;
there was serious damage to the seminiferous epithelium; and a high increment (approximately 25-fold, p <0.0033) in apoptotic germ cells was observed. Thus, Se deficiency exacerbated the toxicity of DEHP on Sertoli cells and
spermatogenesis, whereas Se supplementation provided protection. These results put forward the critical role of Se in the
modulation of redox status of testicular cells and emphasize the importance of Se status for reproductive health. |
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