Effects of ectopic HER-2/neu gene expression on the COX-2/PGE2/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells |
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Authors: | Shu Li XiaoXin Ma Li Ma Cuicui Wang YuanQi He ZhiJuan Yu |
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Institution: | 1.Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, 110004, China |
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Abstract: | ObjectivesTo investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma.MethodsThe recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P450arom in transfected cells were detected by real-time PCR and western blotting. The levels of estrogen in cell supernatants were detected by ELISA.ResultsOver-expression of HER-2/neu in transfected cells was confirmed by real-time PCR and western blotting. The levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of COX-2 and P450arom expression in transfected cells.ConclusionHER-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the COX-2/P450arom signal. |
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Keywords: | HER-2/neu COX-2 P450arom Estradiol Endometrial carcinoma cell |
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