检测受染小鼠血清中循环抗原以诊断旋毛虫病的研究

应用双抗体夹心-ELISA法检测了94只受染小鼠血清中的旋毛虫幼虫循环抗原。受染3d后,即有7％的小鼠显示阳性反应,受染30d后,无论受染幼虫数目多寡(50、150及300条幼虫/只),全部受染鼠(100％)均显示阳性反应。给予丙硫苯咪唑治疗后的第1周,循环抗原的阳性率仍为100％,但在治疗后第2、3及4周,则分别降为60％、20％及10％,表明循环抗原的检测对诊断旋毛虫感染及考核药物疗效起一定作用。

Diagnosis of Trichinosis by Detecting Circulating Antigens in Infected Mice

A total of 94 mice were experimentally infected with Trichinella spiralis larvae, and circulating antigens in their sera were determined at different periods after infection with ELISA double sandwich method, Some infected mice began to show positive reaction 3 days after inoculation and the reaction became strongly positive in all infected animals 30 days post-infection. The percentage of positive reaction and the value of OD in ELISA tended to increase with the increase in intensity and duration of infection, One week after being treated with albendaole, the larvae within the muscles of the infected animals were mostly found dead and some of them appeared to be absorbed by the surrounding tissue, but the reaction still remained 100% positive. However, at 2,3 and 4 weeks after treatment, the percentage of ELISA positives became 60%, 20% and 10%. On the contrary, specific seral antibody titre remained high. It indicates that circulating antigens may differenciate between acute and past infections. Dot-ELISA and reversed indirect hemagglutination were also conducted simultaueously. The positive rates of Sendwich ELISA, Dot-ELISA and RIHA were 76.9%, 56.9% and 33.8% respectively. All control animals showed negative reaction.