丹参和川芎嗪对瘢痕成纤维细胞生长的体外抑制实验

增生性瘢痕是临床治疗难题之一，目前尚无有效的治疗方法。为了探讨丹参和川芎嗪对瘢痕成纤维细胞的抑制作用，取１２例患者胸部增生性瘢痕组织，通过体外成纤维细胞培养，探讨不同药物浓度、不同作用时间对成纤维细胞生长的影响。结果表明，丹参、川芎嗪对体外培养的瘢痕成纤维细胞具有剂量依赖和时间依赖两方面的抑制作用。使细胞胸腺嘧啶核苷的摄入值降低，分裂指数下降，生长增殖减慢甚至停止。在低剂量时，药物对细胞的抑制是可逆的，丹参在２．５ｍｇ／ｍｌ以上，川芎嗪在５００μｇ／ｍｌ７２小时以上，是不可逆的，药物对成纤维细胞的抑制作用主要通过抑制ＤＮＡ合成来实现。为临床应用中草药治疗增生性瘢痕提供了理论依据

THE INHIBITORY EFFECT IN VITRO OF SALVIA MILTORRHIZA AND TETRAMETHYL PYRAZINE ON THE GROWTH OF FIBROBLASTS

In order to investigate the inhibitory effect of salvia miltiorrhiza (SM) and tetramethyl pyrazine (TP) on scartricial fibroblast, the hypertrophic scar tissue of chest was chosen for culture of fibroblasts, and the influence of SM and TP on fibroblasts was observed, The effect of the drugs on the growth of fibroblasts, on DNA synthesis of fibroblasts and on mitosis index of fibroblasts were all determined quantitatively. The results showed: 1. SM and TP could inhibit significantly the growth of the fibroblasts, the inhibitory effect was irreversible when the concentration of the drugs reached 5 mg/ml and 500 micrograms/ml respectively; 2. SM and TP could inhibit the absorption of 3H-TdR and this effect was correlated positively to the dosage of the drugs and; 3. SM and TP could reduce the mitosis index of fibroblasts. It was concluded that SM and TP had definite depressive effect on growth of fibroblasts which was correlated positively with the concentration of drugs and duration of application. The inhibitory effect of the drugs on fibroblasts was mainly through inhibition of synthesis of DNA.