卡托普利对兔动脉粥样硬化斑块局部C反应蛋白表达的影响

目的通过建立兔动脉粥样硬化(AS)模型,观察斑块局部C反应蛋白(CRP)的水平,并观察卡托普利对CRP的影响作用,探讨卡托普利除了抗高血压作用外对血管局部炎症反应的影响。方法雄性健康新西兰兔22只,随机分为3组:①高脂饮食组8只,给予含1%胆固醇+4%猪油饲料;②高脂饮食加卡托普利组8只,饲以1%胆固醇+4%猪油饲料+卡托普利(2mg·kg-1·d-1);③正常对照组6只,饲以标准兔饲料,各组均喂养10周。实验结束后,测各只动物血浆血管紧张素Ⅱ(AngⅡ)水平及血脂;并取主动脉组织作形态学观察并做CRP免疫组织化学分析。结果①高脂饮食组血浆AngⅡ含量与正常对照组相比明显升高(1597±116)pg/mlvs(86±6)pg/ml,P<0.01];高脂饮食加卡托普利组AngⅡ水平较高脂饮食组明显下降,但仍高于正常对照组分别为(386±57)pg/mlvs(1597±116)pg/ml,P<0.01;(386±57)pg/mlvs(86±6)pg/ml,P<0.01];②高脂饮食组斑块局部CRP表达较对照组显著增高(42.1±11.6)%vs(1.8±0.5)%,P<0.01],卡托普利组较高脂饮食组明显下降(23.6±5.3)%vs(42.1±11.6)%,P<0.01];③在卡托普利的干预下,血浆AngⅡ的水平与斑块局部的CRP的表达呈正相关(t=0.673,P<0.01)。结论①卡托普利明显降低斑块局部CRP的表达,减轻斑块局部的炎症反应;②血浆中AngⅡ与AS斑块中CRP的表达呈正相关。

The effects of captopril on the expressions of C-reactive protein in the aortic plaques of atherosclerotic rabbits

Objective To observe the level of plasma angiotensinⅡ(AngⅡ) and local expression of C-reactive protein(CRP) in aortic plaques of atherosclerotic rabbits after intervention by captopril,and to elucidate the anti-inflammatory effects of captopril in addition to antihypertensive action.Methods Twenty-two male rabbits were randomized into three groups:①high-fat diet group,given 1% cholesterol and 4% lard(n=8);② captopril treated high-fat diet group,given 1% cholesterol and 4% lard and treated with 2 mg·kg-1·d-1 captopril(n=8);③ control group,given standardized rabbit food(n=6).After 10 weeks,the levels of plasma AngⅡ and lipids were measured by radioimmuoassay,and the aortas were harvested for pathological study and measurement of CRP by immunohistochemistry.Results ① Compared with control group,the level of plasma AngⅡ was significantly increased in high-fat diet group (1597±116) pg/ml vs(86±6)pg/ml,P<0.01].In captopril-treated high-fat diet group,the level of plasma AngⅡ was decreased significantly than that of high-fat diet group (386±57) pg/ml vs(1597±116) pg/ml,P<0.01],but was nevertheless significantly higher than that of control group (386±57) pg/ml vs(86±6)pg/ml,P<0.01].② Compared with control group,the percentage of CRP-positive cells in plaques were significantly increased in high-fat diet group (42.1±11.6)% vs(1.8±0.5)%,P<0.01].Captopril dramatically reduced the percentage of CRP-positive cells in plaques (23±5)% vs(42±12)%,P<0.01].③ Plasma AngⅡ was positively correlated with the expression of CRP in local atherosclerotic plaques(t=0.673,P<0.01).Conclusion Captopril can reduce the expression of CRP in local plaques of aortic atherosclerosis,and suppress vascular inflammation.Plasma AngⅡ was positively correlated with the expression of CRP in atherosclerotic plaques.