N-乙酰半胱氨酸对乙醇代谢的影响及机制研究

 目的研究N-乙酰半胱氨酸(NAC)是否能通过加速乙醇代谢而解酒以及是否能对抗乙醇对肝脏的损害及其作用机制。方法小鼠随机分组,分别灌胃给予蒸馏水和不同剂量的NAC,20 min后灌胃给予白酒,观察小鼠的翻正反射,攀网能力,用生化比色法测定肝脏中的乙醇脱氢酶(ADH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽硫转移酶(GST)的活性及谷胱甘肽(GSH)和丙二醛(MDA)的含量。用气相色谱法测定血中乙醇的浓度。结果NAC可以减少小鼠灌胃给酒后的醉酒率,延长醉酒的潜伏时间,对抗乙醇引起的攀网能力下降,增加肝脏ADH的活性,从而增加乙醇代谢速度,降低血中乙醇的浓度;增加肝脏SOD,GSH-Px,GST的活性,提高肝脏GSH的含量,有利于清除自由基;减少肝脏中MDA的含量。而且均存在剂量依赖关系。结论NAC能加速乙醇代谢及对抗乙醇的肝脏损害,其作用机制可能与其提高ADH及抗氧化酶活性、加速清除乙醇代谢过程中产生的自由基、减少过氧化脂质的生成有关。

Effects of N-Acetylcysteine on Metabolism of Alcohol and Its Mechanism Research

OBJECTIVE To investigate the effect of N-acetylcysteine on the metabolism of alcohol and the antagonism to the damage of liver caused by alcohol. METHODS The mice were divided into several groups randomly, and were orally given distilled water and different doses of NAC respectively. After 20 min, the mice were orally given alcohol. Then their righting reflex and climb capacity were observed; the activities of ADH, SOD, GST, GSH-Px and the contents of GSH and MDA in liver were measured by chromatometry and colorimetric method. The alcohol concentration in blood was measured by GC. RESULTS NAC significantly reduced the drunk rate of mice after being given alcohol, prolonged their tolerance time, and restrained the decreasing of mice climb capacity induced by alcohol. NAC reduced the blood concentration of alcohol in drunken mice, and increased the activity of ADH, which accelerated the metabolism of the alcohol. NAC increased the activity of SOD, GST, GSH-Px and the content of GSH, which was benefit for ehminating the free radical. NAC reduced the content of the MDA in liver. And all of the above effects of NAC were dose dependent. CONCLUSION NAC has the effect of promoting the metabolism of alcohol and antagonizing the damage of liver caused by alcohol.