含Parvalbum in和Calbind in D-28k样免疫阳性神经元在大鼠三叉神经本体觉中枢通路的分布

本研究应用免疫组织化学技术对Ｐａｒｖａｌｂｕｍ ｉｎ（ＰＶ）和Ｃａｌｂｉｎｄｉｎ Ｄ ２８ｋ（ＣＢ）样免疫阳性神经元在大鼠三叉神经本体觉中枢通路上的分布状况作了普查。结果表明： 这两种钙结合蛋白在该通路上具有下列的分布特点：（１）三叉神经中脑核神经元几乎全部为ＰＶ 样免疫阳性，胞体和突起染色强或中等强度，胞体大（１８～３０ μｍ ），多为假单级神经元，偶见中等大小的多极神经元。（２）在三叉神经脊束核吻侧亚核尾侧段水平的三叉神经脊束核吻侧亚核背内侧部及邻接的网状结构中可看到由ＰＶ 样免疫阳性神经元构成的两团深染区，外侧者大，内侧者小，胞体呈圆形、三角形或不规则形（９～１６ μｍ ）；在此处ＣＢ样免疫阳性细胞亦呈类似分布。（３）“带状区”内ＰＶ 样免疫阳性细胞多为具有短突的多极神经元；在三叉上核尾外侧部，ＰＶ 样神经元分布稀疏，但在感觉主核背内侧部则呈高密度分布；ＡＶＭ 、ＡＤＯ 等两个位于“带状区”腹侧部的新发现的小核团亦可观察到阳性细胞和突起。此“带状区”内ＣＢ样免疫阳性细胞数目较少，分布也稀疏，但胞体较大（１１～１８ μｍ ），呈三角形、圆形或不规则形，轴突上可见少量的串珠状膨体。在ＡＤＯ 中ＣＢ样免疫阳性细胞的分布较Ｐ?

DISTRIBUTION OF PARVALBUMIN AND CALBINDIN D 28k LIKE IMMUNOREACTIVE NEURONS IN THE CENTRAL PATHWAY OF TRIGEMINAL PROPRIOCEPTION OF THE RAT

In the present study, the distribution of parvalbumin(PV) and calbindin D 28k(CB) Like immunoreactivities( LI) in the central pathway of trigeminal proprioception of the rat was examined by the immunohistochemical method. The results indicated that PV and CB LI in the pathway showed the following distribution characteristics. (1) Nearly all neurons in the mesencephalic trigeminal nucleus(Vme) showed PV LI. Both the cell bodies and the processes displayed high to moderate immunostaining. Most neurons of PV LI were large (18～30 μm), pseudounipolar neurons; medium sized multipolar ones could be occasionally seen. (2) At the level of caudal part of the subnucleus oralis of the spinal trigeminal nucleus often occurred two well separated areas of high intensity of PV LI in the dorsomedial part of the subnucleus oralis of the spinal trigeminal nucleus and its adjacent lateral reticular formation(Vodm LRF): one was large, the other was small; and the cell bodies were round, triangular or irregular in shape(9～16 μm). Neurons with CB LI in Vodm LRF were multipolar with different size and shape, and showed similar distribution pattern to that of PV. (3) PV LI neurons were multipolar with short processes in the “zone shaped area”. In the caudolateral part of supratrigeminal nucleus (Vsup CL), PV LI neurons were sparsely distributed. However, a large number of neurons in the whole dorsomedial part of the principal sensory trigeminal nucleus (Vpdm) expressed PV LI. A few cell bodies and processes in the two newly found nuclei, the area ventral to the motor trigeminal nucleus (AVM) and the area dorsal to the superior olivary nucleus (ADO), were also labeled. By comparison, the neurons displaying CB LI in these nuclei were smaller in number and sparsely distributed, they were round, triangular or irregular in shape and larger in size(11～18 μm),and beaded varicosities could be easily seen along the axons. In ADO the distribution region of CB LI neurons was slightly more dorsomedial than that of PV LI. The present results indicated that the two main calcium binding proteins, PV and CB, were widely distributed in the central pathway of the trigeminal proprioception of the rat, and suggested that PV and CB might play important roles in the transmission of the trigeminal proprioceptive information. (Figures A F on plate 50)