| 多核白细胞弹性蛋白酶对人角膜基质细胞MMP-2、MMP-9表达的影响 |
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| 引用本文： | 刘泊明,张冰洁,杨贲,郝继龙.多核白细胞弹性蛋白酶对人角膜基质细胞MMP-2、MMP-9表达的影响[J].中国实验诊断学,2010,14(6):855-857. |
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| 作者姓名： | 刘泊明 张冰洁 杨贲 郝继龙 |
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| 作者单位： | 1. 伊通满族自治县第一人民医院,眼科,吉林,伊通,130700
2. 吉林大学中日联谊医院,眼科,吉林,长春,130033 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的观察多核白细胞弹性蛋白酶与白细胞介素-1α对人角膜基质细胞表达的MMP-2、MMP-9的影响,进而探讨角膜溃疡的发生机理。方法体外培养人角膜基质细胞,在培养液中添加或不添加多核白细胞弹性蛋白酶或白细胞介素-1α,培养5天,通过明胶酶谱分析的方法测定培养上清中的MMP-2和MMP-9的表达。结果体外培养的人角膜基质细胞无任何刺激下可释放MMP-2;白细胞介素-1α可加强并激活MMP-2的表达,同时可诱导MMP-9前体的释放;多核白细胞弹性蛋白酶在白细胞介素-1α诱导下可完全激活前体MMP-9转化为活化形式。结论多核白细胞弹性蛋白酶和白细胞介素-1α协同作用参与角膜溃疡的发生。
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| 关 键 词： | 多核白细胞弹性蛋白酶 白细胞介素-1α 角膜基质细胞 MMP-2 MMP-9 |
Effects of neutrophil elastase on expression of the MMP - 2,9 in cultured human corneal fibroblasts |
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| Institution: | LIU Bo-ming, ZHANG Bing-jie, YANG Ben, et al. ( 1. Department of Ophthalmology, The first people＇ s hospital of Yitong Manchu Autonomous County, Jilin Yitong 130700, China ;2. Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130033, China) |
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| Abstract: | Objective To understand the mechanism of corneal ulceration, we investigated the role of neutrophil elastase （NE）, interleukin-1α（IL-1α） and corneal fibroblasts on expression of the matrix metalloproteinases（ MMP）-2,9 in cultured human corneal fibroblasts. Methods NE or IL-1α was added to the culture medium of human corneal fibroblasts 5 day later. The activity of MMP-2 and MMP-9 were examined by Gelatin zymography. Results Cultured human corneal fibroblasts released MMP-2 without any stimulation. IL-1α enhanced and activated the expression of MMP-2, while induced the release of proMMP-9. NE transformed proMMP-9into active fomts induced by IL-1α. Conclusion NE and IL-lacan promote human corneal fibroblasts in collagen degradation,the two are synergistic. |
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| Keywords: | MMP-2 MMP-9 |
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