HPLC测定洛伐他汀烟酸缓释片中两组分含量

 目的建立洛伐他汀烟酸缓释片中2个组分(洛伐他汀、烟酸)含量的高效液相色谱法。方法采用Hypersil C₁₈色谱柱(4.6 mm×250 mm,5μm),以0.005 mol·L^-1己烷磺酸钠溶液-乙腈(35:65)为流动相,检测波长为239 nm。结果烟酸和洛伐他汀的浓度分别在49.8～249和2～10 mg·L^-1有良好的线性关系(r=0.999 6),样品的回收率分别为99.93%(n=6,RSD=0.73%)和101.3%(n=6,RSD=1.61%)。结论本法简便、快速,重现性好,灵敏度高,适用于洛伐他汀烟酸缓释片的质量控制。

Determination of two components in lovastatin and niacin sustained release tablets by HPLC

OBJECTIVE To establish a HPLC method for the determination of lovastatin and niacin in lovastatin-niacin sustained release tablets .METHODS The separation was performed on a C₁₈ column(4.6 mm×250 mm,5 μm) with 0.005 mol·L^-1 sodium 1-hexanesul-fonate-acetonitrile(35:65)as the mobile phase.The monitoring wavelength was 239 nm.RESULTS The linear ranges were 49.8-249 and 2-10 mg·L^-1(r=0.999 6),the recoveries were 99.93%(n=6, RSD=0.73%) and 101.3%(n=6, RSD= 1.61%)for niacin and lovastatin, respectively.CONCLUSION The method was sensitive, specific, simple, and suitable for the quality control of lovastatin-niacin sustained-release tablets.