| 紫外-可见分光光度法测定双黄连颗粒中黄芩苷的含量 |
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| 引用本文： | 韩秋伟. 紫外-可见分光光度法测定双黄连颗粒中黄芩苷的含量[J]. 牡丹江医学院学报, 2012, 33(2): 21-23 |
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| 作者姓名： | 韩秋伟 |
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| 作者单位： | 黑龙江林业职业技术学院 黑龙江牡丹江 157011 |
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| 摘 要： | 目的：建立双黄连颗粒中黄芩苷的含量测定方法。方法：采用紫外-可见分光光度法。以90%的乙醇溶液和0.2mol/L盐酸溶液为溶剂,检测波长为276nm。结果：回归方程为A=0.06250006C＋0.019683333（r=0.9992）,黄芩苷在6.0～30.0μg/mL浓度范围内呈良好的线性关系,平均回收率为100.18%,RSD为0.88%。结论：该方法简便快速、重现性好,结果准确可靠,可作为一种快速测定双黄连颗粒中黄芩苷含量的分析方法。
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| 关 键 词： | 紫外-可见分光光度法 双黄连颗粒 黄芩苷 含量测定 |
THE CONTENT OF BAICALIN OF SHUANGHUANGLIAN GRANULES DETERNINED BY ULTRAVIOLET- VISIBLE SPECTROPHOTOMETRY |
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| Han Qiu wei. THE CONTENT OF BAICALIN OF SHUANGHUANGLIAN GRANULES DETERNINED BY ULTRAVIOLET- VISIBLE SPECTROPHOTOMETRY[J]. Journal of Mudanjiang Medical College, 2012, 33(2): 21-23 |
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| Authors: | Han Qiu wei |
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| Affiliation: | Han Qiu wei(Heilongjiang Linyezhiyejishuxueyuan,Mudanjiang,157011,China) |
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| Abstract: | Objective:The purpose is to develop a method to determine the content of Baicalin in shuanghuangliang granules.Methods:To adopt the ultraviolet spectrophotometry.The solvent is 90% alcohol solution and 0.2mol/L HCl solution.Detection is 276nm.Results::Regression equation:A=0.06250006C+0.019683333(r=0.9992),the linear relationship between the Baicalin content and absorbability is good when the Baicalin concentration range is 6.0～30.0μg/mL.The average recovery was 100.18% and RSD is 0.88%.Conclusion:The method is simple,quick and reproducible.The result is correct and reliable.It can be used as the fast methed to determine content of Baicalin in shuanghuanglian granules. |
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| Keywords: | Ultraviolet-visible spectrophotometry shuanghuanglian granules Baicalin the content determine |
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