流式细胞术检测类风湿关节炎滑液对中性粒细胞凋亡的影响

目的观察骨关节炎(OA)和类风湿关节炎(RA)患者滑液对中性粒细胞凋亡的影响。方法通过流式细胞仪及荧光显微镜检查19例RA和11例OA患者对体外培养粒细胞凋亡的影响。结果流式细胞仪及荧光显微镜检测的体外培养粒细胞自发凋亡发生率分别为43.6%和37.4%,加RA滑液组分别为18.3%和13.7%(P<0.01),加OA滑液组则分别为40.3%和31.2%(P>0.05)。结论RA滑液可显著降低粒细胞凋亡发生率,流式细胞仪检测凋亡较荧光显微镜检查更具敏感性。

Flow Cytometry Analysed the Effect of Synovial Fluids from Rheumatoid Arthritis on Neutrophil Apoptosis

Objective To investigate neutrophil apoptosis in the presence of synovial fluids(SF) from patients with rheumatoid arthritis(RA)and osteoarthritis(OA).Methods Neutrophil apoptosis was determined by flow cytometric analysis of annexin-V binding,and by terminal transferase-mediated dUTP-fluorescensin nick end-labeling(TUNEL) technique in the presence or absence of synovial fluids from 19 RA patients and 11 OA patients.Results In the presence of RA SF,the rate of neutrophil apoptosis was reduced from 43.6% to 18.3%(P<0.01) as detected by flow cytometric analysis;and from 37.4% to 13.7%(P<0.01) as assayed by TUNEL method.Whereas,no significant change of neutrophil apoptosis was observed in the persence of OA SF.Conclusion This study indicates that RA SF is capable of slowing the rate of neutrophil apoptosis,and that flow cytometric analysis of annexin-V binding is more sensitive than TUNEL method in detection of cell apoptosis.