亚洲牛带绦虫Spef1-Like基因及其蛋白结构特征的生物信息学分析

目的分析和预测亚洲牛带绦虫的Spef1-Like基因及其编码的蛋白质的结构和功能。方法利用生物信息学网站如美国国家生物技术中心(NCBI,http://www.ncbi.nlm.nih.gov/)和瑞士生物信息学研究所的蛋白分析专家系统(Ex-PASY,http://ca.expasy.org/)所提供的有关基因和蛋白序列和结构功能分析工具,以及专业的生物信息学软件包如pc-gene,VectorNTIsuite,从亚洲牛带绦虫成虫cDNA文库当中识别该基因及编码区,分析和预测该基因编码的蛋白质的理化性质,一级结构中的修饰位点及模序,亚细胞定位特征,二级结构特征以及蛋白三维空间构象,蛋白亲水性及潜在抗原表位。结果该基因全长1099bp,编码区为117-929bp,编码271个氨基酸,与GenBank当中的其他序列比对,预测该基因为全长基因。编码的蛋白质理化性质比较稳定,含有多个能被其他酶修饰的位点,无跨膜区,预测其主要含有三个亲水性较强的抗原表位,空间结构上相距较近。结论生物信息方法可从亚洲牛带绦虫成虫cDNA文库中筛选出Spef1-like基因并进行分析。

Informatics analysis for the structure and function of spef1-like gene from Taenia saginata asiatica

To analyze and predict the structural characteristics of Spef1 Like gene from Taenia saginata asiatica by bioinformatics.By using the bioinformatics tools in bioinformatics webs site such as NCBI(http://www.ncbi.nlm.nih.gov/),ExPASY(http://www.expasy.org/) and the others the particular packages of biofomatic software as pcgene and vector NTI suite 8.0.The Spef1-Like gene and the coding region sequence from Taenia saginata asiatica cDNA club were firstly identifed,then the characteristics of structure and fuction of Spef1-like protein were analyzed and predicated using two ways mentioned above,including physical and chemical characteristics,modified sites and motifs in first structure,subcellular location,secondary structure and formation in three dimesion space,hydropathicity and potential epitopes and so on.This gene was 1099bp in length and its coding site was from 117bp to 929bp,coding 271 amina acids,aligxed with other genes from GenBank and was a complete full-length gene.The protein deduced from the gene was stable at physical and chemical characteristics,owning many potential modiified sites and the over-membrance region.Three major linear epitope were nearer from each other on the surface of spatial structure of Spef1-like gene.It is concluded that Spef1-like gene was screened from the cDNA library of adult Taenia asiatica by bioinformatical method.