多种肿瘤标志物蛋白质芯片在原发性肝癌检测中的应用

目的: 研究多种肿瘤标志物蛋白芯片诊断系统(C12)12种肿瘤标志物的联合检测在原发性肝癌诊断中的价值。方法: 用C12定量检测128例原发性肝癌患者、23例肝硬化患者和98名正常人血清的12种常见的肿瘤标志物,包括糖链抗原125(CA 125)、血清铁蛋白(Ferritin)、癌胚抗原(CEA)、甲胎蛋白(AFP)、糖链抗原19-9(CA19-9)、糖链抗原15-3(CA15-3)、神经元特异性烯醇化酶(NSE)、糖链抗原242(CA242)、前列腺特异性抗原(PSA)、游离前列腺特异性抗原(Free-PSA)、人类生长激素(HGH)、人绒毛膜促性腺激素(β-hCG)。结果: 通过多指标联合检测,128例原发性肝癌患者血清肿瘤标志物中有112例肿瘤标志物阳性,灵敏度从AF P单指标测定的58.6%提高到87.5%,特异性达83.5%;ferritin阳性率达41.4%,原发性肝癌患者血清中β-hCG、NSE、PSA、HGH、CA19-9、CA125、CEA、AF P、CA15-3、CA242与正常对照组和肝硬化组比较差异均有显著性(P<0.01)。结论: 联合检测与传统的单指标检测相比灵敏度大大提高。

Application of protein chip system to the detection of primary liver cancer

Objective: To study the value of 12 tumor markers in protein chip system for multi-tumor detection(C12) in the diagnosis of the primary liver cancer.Methods: The system of C12 was used to quantitatively detect the 12 tumor markers(CA125,Ferritin,CEA,AFP,CA19-9,CA15-3,NSE,CA242,PSA,Free PSA,HGH,β-hCG) in the sera of 128 patients with primary liver cancer,23 patients with hepatocirrhosis,and 98 healthy controls.Results: The quantitative detection proved positive in 112 of the 128 patients with primary liver cancer;the sensitivity was raised from 58.6% by AFP single index detection to 87.5% and the specificity reached 83.5%.In addition,Ferritin positive rate achieved 41.4%,and in the sera of some liver cancer patients,the difference of β-hCG,NSE,PSA,HGH,CA19-9,CA125,CEA,AFP,CA15-3 and CA242 items was statistically significant,compared with the contrast group and hepatocirrhosis group(P<0.01).Conclusions: The allied detection is more effective than the traditional single index detection.