GC-MS代谢组学分析逍遥散干预抑郁模型大鼠盲肠代谢物组的变化规律

目的采用GC-MS代谢组学方法分析逍遥散干预抑郁模型大鼠盲肠组织代谢物组的变化规律。方法采用慢性温和不可预知应激(CUMS)程序对大鼠进行造模,以逍遥散及盐酸氟西汀(氟西汀)为干预药物,采用GC-MS方法分析大鼠盲肠组织代谢物组及其变化规律。化合物的结构通过NIST 05数据库检索及对照品指认确定,将数据预处理后导入SIMCA-P 13.0软件进行多元统计分析。结果 CUMS抑郁大鼠模型复制成功,代谢组学研究结果表明氟西汀及逍遥散干预后大鼠盲肠组织中丙氨酸、丝氨酸、谷氨酸等减少,氟西汀组棕榈酸、硬脂酸升高,且与模型组比较具有显著性差异(P0.05、0.01)。结论逍遥散干预后CUMS大鼠盲肠组织的代谢物组发生明显变化,该研究可为逍遥散抗抑郁作用机制研究提供依据。

Analysis on endogenous metabolites in cecal tissue of CUMS-induced depression rats after Xiaoyao San intervention by GC-MS metabolomics

Objective To investigate the changes of endogenous metabolites in cecal tissue of CUMS-induced depression rat model interfered by Xiaoyao San via GC-MS metabolomics. Methods Chronic unpredictable mild stress (CUMS) rat model was used and Xiaoyao San and fluoxetine hydrochloride (fluoxetine) treatment were intervention drug. The endogenous metabolites were analyzed and identified by NIST 05 database or authentic standards. The final data were introduced into SIMCA-P 13.0 software package for multivariate analysis. Results The CUMS rat model was successfully duplicated, the metabolites of alanine, serine, and glutamic acid were reduced at fluoxetine group and Xiaoyao San group, and palmitic acid and stearic acid were increased in fluoxetine group in cecal tissue of rats after drug intervention, compared with model group with significant difference (P < 0.01 or 0.05). Conclusion The endogenous metabolites were changed by Xiaoyao San intervention significantly in cecal tissue of CUMS rats, which provides a basis for further study of the mechanism of Xiaoyao San treatment of depression.