| 亲和色谱分离纯化低分子肝素 |
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| 引用本文： | 何书英,李华,杨宁. 亲和色谱分离纯化低分子肝素[J]. 中国生化药物杂志, 2006, 27(3): 170-171 |
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| 作者姓名： | 何书英 李华 杨宁 |
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| 作者单位： | 中国药科大学,生命科学与技术学院,江苏,南京,210009 |
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| 摘 要： | 目的探索亲和色谱纯化低分子肝素的方法。方法以壳聚糖为载体,以抗凝血酶Ⅲ及凝血酶为配基分别制备亲和吸附剂,采用亲和方法分离纯化低分子肝素,并对两者的纯化效果进行比较。结果前者制备的低分子肝素抗Ⅹa因子活性为173.9 IU/mg,比纯化前提高1.7倍;后者制备的低分子肝素抗Ⅹa因子活性为646.0 IU/mg,比纯化前提高6.3倍。结论经壳聚糖-抗凝血酶Ⅲ纯化的低分子肝素其抗Ⅹa因子活性显著提高。
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| 关 键 词： | 壳聚糖 凝血酶 抗凝血酶Ⅲ 低分子肝素 亲和色谱 |
| 文章编号： | 1005-1678（2006）03-0170-02 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-12-09 |
Purification of the low molecular weight heparin by affinity chromatography |
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| HE Shu-ying,LI Hua,YANG Ning. Purification of the low molecular weight heparin by affinity chromatography[J]. Chinese Journal of Biochemical Pharmaceutics, 2006, 27(3): 170-171 |
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| Authors: | HE Shu-ying LI Hua YANG Ning |
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| Affiliation: | School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China |
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| Abstract: | Purpose To study purification method of the low molecular weight heparin(LMWH) by affinity chromatography.MethodsChitosan-thrombin and chitosan-antithrombinⅢ adsorbent were prepared respectively.The effects of above two affinity adsorbents were compared through the anti-FⅩa activity of purified LMWH.ResultsIt showed that the anti-FⅩa activities of the LMWH purified through chitosan-thrombin adsorbent and chitosan-antithrombinⅢ adsorbent were 173.9 U/mg and 646.0 U/mg,which raised 1.7 times and 6.3 times respectively compared with the unpurified LMWH.ConclusionThe anti-FⅩa activity of LMWH purified through chitosan-antithrombinⅢ adsorbent was evidently elevated. |
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| Keywords: | chitosan thrombin anti-thrombinm low molecular weight heparin affinity chromatography |
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