| 苯乙酸钠对肺癌A549细胞生长的影响 |
| |
| 引用本文： | 张莉,李清泉,杨炯. 苯乙酸钠对肺癌A549细胞生长的影响[J]. 武汉大学学报(医学版), 2006, 27(2): 249-252 |
| |
| 作者姓名： | 张莉 李清泉 杨炯 |
| |
| 作者单位： | 武汉大学人民医院呼吸内科,武汉,430060 |
| |
| 摘 要： | 目的:研究抗肿瘤药物苯乙酸钠(NaPA)对体外培养的人肺腺癌细胞株A549抑制率、细胞周期和凋亡的影响。方法:应用四甲基偶氮唑蓝(MTT)比色、流式细胞仪等方法,观察不同浓度NaPA对人肺腺癌A549细胞株生长、细胞周期和凋亡的影响。结果:NaPA对A549有明显的生长抑制作用,且呈剂量时间依赖性,最大抑制率可达79%。NaPA可阻滞A549细胞的细胞周期,使G1/G0期细胞增多,S期细胞减少,同时诱导A549细胞的凋亡,凋亡率最高可达30.5%。结论:苯乙酸钠抑制A549细胞生长,其抑制作用可能是通过NaPA使细胞周期抑制于G1/G0期和诱导细胞凋亡而实现。
|
| 关 键 词： | 苯乙酸钠 细胞周期 凋亡 人肺腺癌A549细胞 |
| 文章编号： | 1671-8852(2006)02-0249-04 |
| 收稿时间： | 2005-09-26 |
| 修稿时间： | 2005-09-26 |
Effect of Sodium Phenylacetate on Cell Growth of Human Lung Adenocarcinoma Cells |
| |
| ZHANG Li,LI Qingquan,YANG Jiong. Effect of Sodium Phenylacetate on Cell Growth of Human Lung Adenocarcinoma Cells[J]. Medical Journal of Wuhan University, 2006, 27(2): 249-252 |
| |
| Authors: | ZHANG Li LI Qingquan YANG Jiong |
| |
| Affiliation: | Dept. of Respiratory, Renmin Hospital of Wuhan University, Wuhan 430060, China |
| |
| Abstract: | Objective: To investigate the effect of sodium phenylacetate (NaPA) on lung adenocarcinoma cell line A549. Methods: Time and dose effects of NaPA were deceted using MTT color metric assay and the influences in the cell cycle and the apoptosis were observed through flow cytometry. Results: NaPA could inhibit the growth of A549 cell line in a dose-depended manner. The biggest rate of cell growth inhibition was up to 79% in the those treated with NaPA. The cytometry confirmed that NaPA could arrest the lung cancer cells from G_ 0/G_ 1 to S stage and induce A549 cells apoptosis. Conclusion: NaPA can inhibit A549 cell line, and the mechanism may be related to the influence in the cell cycle and induction of apoptosis. |
| |
| Keywords: | Sodium Phenylacetate Cell Cycler Apoptosis Human Lung Adenocarcinoma Cell Line |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
