苯妥英钠对慢性应激大鼠海马CA3区锥体神经元形态结构的影响

目的:研究苯妥英钠对慢性应激大鼠海马CA3区锥体神经元形态结构的影响。方法:采用Nissl染色,Golgi染色及电子显微镜技术观察各组大鼠海马CA3区锥体神经元的形态结构。结果:慢性应激引起了海马CA3区神经元的丢失(由39±4减少至35±4)、顶树突总长度的缩短(由196μm±35μm缩短至156μm±33μm,P<0.05),以及神经元超微结构的变性。苯妥英钠显著抑制了慢性应激所致海马CA3区神经元的减少(38.4±2.2)及顶树突总长度的缩短(198μm±36μm,P<0.05),改善了神经元超微结构的变性。结论:慢性应激可导致大鼠海马CA3区锥体神经元损伤,苯妥英钠对应激状态下的海马CA3区锥体神经元具有保护作用。

Effects of phenytoin on morphology and structure of hippocampal CA3 pyramidal neurons of rats in chronic stress

AIM: To investigate the effects of phenytoin (DPH) on morphological and structural changes of pyramidal neurons in hippocampal CA3 of rats induced by chronic stress. METHODS: Using Nissl staining, Golgi staining, and electron microscope, the morphology and structure of pyramidal neurons in hippocampal CA3 of rats were observed. RESULTS: Chronic stress resulted in loss of hippocampal CA3 pyramidal neuron from 39+/-4 to 35+/-4, shortening of total length of apical dendrite (from 196 microm+/-35 microm to 156 microm+/-33 microm, P<0.05), and ultrastructural degenerations of neurons. DPH markedly inhibited the decreases in number of hippocampal CA3 pyramidal neuron (38.4+/-2.2) and total length of apical dendrite (198 microm+/-36 microm, P<0.05), meanwhile, improved neuron ultrastructural degenerations caused by chronic stress. CONCLUSION: Chronic stress does damage to hippocampal CA3 pyramidal neurons and DPH protects hippocampus from damage induced by chronic stress.