| 乳腺癌MCF-7肿瘤细胞SP亚群初步分析 |
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| 引用本文： | 张玥,张凤春,张雁云.乳腺癌MCF-7肿瘤细胞SP亚群初步分析[J].上海交通大学学报(医学版),2006,26(5):507-510. |
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| 作者姓名： | 张玥 张凤春 张雁云 |
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| 作者单位： | 吉林大学中日联谊医院血液肿瘤科，上海交通大学医学院仁济医院肿瘤中心，中国科学院上海生命科学院健康科学中心免疫学联合实验室 长春130033 |
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| 基金项目： | 上海交通大学医学院肿瘤协作研究中心资助项目(ZL200503) |
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| 摘 要： | 目的鉴定乳腺癌干细胞系MCF-7中是否包含肿瘤干细胞相关的SP(side popu lation)亚群。方法制备MCF-7细胞悬液,经Hoechst33342和PI染色(其中一组同时加入拮抗剂维拉帕米作为对照),流式细胞仪分析SP亚群。另取贴壁生长的MCF-7细胞,用Hoechst33342和羟基荧光素二醋酸盐琥珀酰亚胺脂(CFSE)原位染色,荧光显微镜下观察细胞染色情况。结果SP亚群占MCF-7细胞的4%,维拉帕米阻断后减少为0.5%。荧光显微镜下见Hoechst33342阳性细胞约占95%。结论人乳腺癌细胞系MCF-7中存在SP亚群,即提示乳腺癌干细胞的存在;维拉帕米可抑制染料外排而减少SP细胞的比例。
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| 关 键 词： | 乳腺癌 肿瘤干细胞 MCF-7细胞 SP亚群 |
| 文章编号： | 0258-5898(2006)05-0507-04 |
| 收稿时间： | 2005-09-29 |
| 修稿时间： | 2005年9月29日 |
Analysis of Side Population in Human Breast Cancer Cell Line MCF-7 |
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| ZHANG Yue,ZHANG Feng-chun,ZHANG Yan-yun.Analysis of Side Population in Human Breast Cancer Cell Line MCF-7[J].Journal of Shanghai Jiaotong University:Medical Science,2006,26(5):507-510. |
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| Authors: | ZHANG Yue ZHANG Feng-chun ZHANG Yan-yun |
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| Institution: | 1.Department of Hematology and Oncology, China-Japan Union Hospital of Jilin University, Changchun 130033, China; 2.Center for Oncology , Renji Hospital, School of Medicine, Shanghai Jiaotong University; 3.Joint Laboratory of Immunology, Health Science Center, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences |
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| Abstract: | Objective To identify the side population(SP) of cancer stem-like cells in breast cancer cell line MCF-7. Methods MCF-7 tumor cells suspension was stained with Hoechst33342 and PI in the absence or presence of verapamil.Then the SP was analyzed in the fluorescence-activated cell sorter.The attached MCF-7 cells cultured in dish were examined by fluorescence microscopy after being stained with Hoechst33342 and carboxyfluorescein diacetate succinimidyl ester. Results SP cells accounted for 4% of the MCF-7 breast cancer cells,which were decreased to 0.5% by treating with verapamil.95% Hoechst33342 positive cells were detected by a fluorescence microscope. Conclusion Breast cancer cell line MCF-7 contains SP which can be decreased by verapamil. |
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| Keywords: | breast cancer tumor stem cells MCF-7 cells side population |
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