肿瘤转移相关基因mts1及nm23-H1在肺癌中的表达及其与淋巴结转移的关系

目的探讨转移相关基因mts1及nm23-H1在人肺癌中的表达及其与淋巴结转移的关系.方法对手术切除后新鲜的26例肺鳞癌和13例肺腺癌组织标本采用地高辛标记的mts1及nm23-H1 cDNA探针进行原位杂交.结果26例鳞癌中,淋巴结转移组11例,mts1 mRNA阳性表达9例,阳性率82%,nm23-H1 mRNA阳性表达8例,阳性率73%;无淋巴结转移组15例,mts1 mRNA阳性表达4例,阳性率27%,nm23-H1 mRNA阳性表达10例,阳性率67%.13例肺腺癌中,淋巴结转移组5例,mts1 mRNA阳性表达4例,阳性率80%,nm23-H1 mRNA阳性表达4例,阳性率80%;无淋巴结转移组8例,mts1 mRNA阳性3例,阳性率38%,nm23-H1 mRNA阳性表达6例,阳性率75%.结论mts1 mRNA表达在肺鳞癌的淋巴结转移与非转移组之间相差显著,在肺腺癌的淋巴结转移组与非转移组之间差异性无统计学意义;nm23-H1 mRNA表达在肺鳞癌、腺癌的淋巴结转移组与非转移组之间差异性不显著.

Tumor metastasis associated gene mts1 and nm23-H1: expression in the lung carcinoma and relationship with lymph node metastasis

Objective To study the expression of tumor metastasis associated gene mts1 and nm23-H1 in the human lung carcinoma and their relationship with lymph node metastasis. Methods Fresh specimens excised from 26 cases of human lung squamous cell carcinoma and 13 cases of human lung adenocarcinoma were hybridized in situ with mts1 and nm23-H1 cDNA probes marked with digoxin. Results In 26 cases of human lung squamous cell carcinoma, there were 11 cases of lymph node metastasis, including 9 cases of positive mts1 mRNA expression (82%) and 8 cases of positive nm23-H1 mRNA expression (73%), and 15 cases of non-lymph node metastasis, including 4 cases of positive mts1 mRNA expression (27%) and 10 cases of positive nm23-H1 mRNA expression (67%). In 13 cases of human lung adenocarcinoma, there were 5 cases of lymph node metastasis, including 4 cases of positive mts1 mRNA expression (80%) and 4 cases of positive nm23-H1 mRNA expression (80%), and 8 cases of non-lymph node metastasis, including 3 cases of positive mts1 mRNA expression (38%) and 6 cases of positive nm23-H1 mRNA expression (75%). Conclusion mts1 mRNA expression in lung squamous carcinoma was significantly different between lymph node metastasis group and non-lymph node metastasis group and that in lung adenocarcinoma was of no difference between two groups. nm23-H mRNA expression in lung squamous carcinoma or in lung adenocarcinoma was of no significant difference between two groups.