| 瘦素受体在实验性多囊卵巢综合征大鼠卵巢中的表达及其意义 |
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| 引用本文： | 苏艳华,王雪金.瘦素受体在实验性多囊卵巢综合征大鼠卵巢中的表达及其意义[J].福建医科大学学报,2009,43(1):18-22. |
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| 作者姓名： | 苏艳华 王雪金 |
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| 作者单位： | 1. 福建医科大学附属协和医院,妇产科,福州,350001
2. 福建医科大学附属第二医院,妇产科,泉州,362000 |
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| 基金项目： | 福建省卫生厅青年基金 |
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| 摘 要： | 目的研究来曲唑诱导建立多囊卵巢综合征（PCOS）动物模型中卵巢组织瘦素受体（Ob-R）及其mRNA的表达，探讨卵巢Ob-R变化与PCOS发病机制的关系。方法6周龄清洁级雌性SD大鼠45只，分为正常组、溶剂组和来曲唑组，各15只。正常组每日自由摄取食物和水；溶剂组每日给予l％羟甲基纤维素水溶液2mL／kg灌胃；来曲唑组以来曲唑0．5mg／kg灌胃。观察大鼠体质量、动情周期、卵巢质量及其形态学改变，测定血清黄体生成素（LH）、睾酮（T）、胰岛素（INS）、瘦素（LEP）及血糖（FG）水平，采用免疫组织化学和RealTimeRT-PCR方法检测大鼠卵巢中Ob-R及其mRNA的相对表达，并对3组的各指标进行比较。结果来曲唑组大鼠体质量和卵巢质量指数升高，动情周期消失，卵巢体积增大，白膜增厚，H—E染色可见较多囊状扩张的卵泡且黄体数量明显下降，血清T、LH、INS、LEP水平明显升高（P〈0．05）；卵巢Ob-R及其mRNA表达明显增强（P〈0．05）。结论来曲唑诱导建立的PCOS动物模型临床表现与人类似；卵巢中Ob—R表达上调，可能是导致PCOS患者不排卵、生育力下降的原因之一。
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| 关 键 词： | 多囊卵巢综合征 卵巢 瘦素 受体 肽 疾病模型 动物 |
Expression of Leptin Receptor in Ovaries of Rat Model with Polycystic Ovarian Syndrome |
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| SU Yanhua,WANG Xuejin.Expression of Leptin Receptor in Ovaries of Rat Model with Polycystic Ovarian Syndrome[J].Journal of Fujian Medical University,2009,43(1):18-22. |
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| Authors: | SU Yanhua WANG Xuejin |
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| Institution: | SU Yanhua 1,WANG Xuejin21.Department of Gynecology and Obstetrics,The Affiliated Union Hospital,Fujian Medical University,Fuzhou 350001,China,2.Department of Obstetrics and Gynecology,The Affiliated Second Hospital,Quanzhou 362000 |
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| Abstract: | Objective To investigate leptin receptor in the ovaries of rat model with polycystic ovarian syndrome(PCOS) induced by Letrozole,in relation to pathogenesis of PCOS.Methods Forty-five female SD rats of 6 weeks old were divided into normal group,solvent group and letrozole group.The letrozole group was gavaged with Letrozole(0.5 mg/kg).Observation and comparison for body weight,estrous cycle,ovarian weight and ovarian morphological changes were conducted.Serum T,LH,FG,INS,and LEP level were measured,The rela... |
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| Keywords: | polycystic ovary syndrome ovary leptin receptors peptide disease models animal |
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