| 人卵巢癌裸鼠移植瘤和腹水瘤模型的建立及形态学观察 |
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| 引用本文： | 陈爱平,王和,彭芝兰,高国兰,白绍槐. 人卵巢癌裸鼠移植瘤和腹水瘤模型的建立及形态学观察[J]. 肿瘤, 2001, 21(2): 82-84 |
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| 作者姓名： | 陈爱平 王和 彭芝兰 高国兰 白绍槐 |
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| 作者单位： | 1. 现在青岛大学医学院附属医院妇产科;华西医科大学附二院妇产科
2. 华西医科大学附二院妇产科, |
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| 基金项目： | 国家自然科学基金资助(批准号39470721) |
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| 摘 要： | 目的 建立人卵巢癌裸鼠移植模型，为研究人卵巢癌发病机制、实验治疗提供工具。方法 采用组织学完整 的人卵巢癌实体瘤和腹水瘤标本植入裸鼠腹腔和皮下，观察成瘤生长和转移以及形态学特征（光镜、电和染色体）。结果 10例卵巢癌标本2例实体瘤、1例腹水瘤移植成功，实体瘤传至第六代，腹水瘤传至第十八代，传代移植成功率100％，移植瘤病理学和电镜观察、染色体核型分析、肿瘤标记物检查均与人体原发肿瘤一致。结论 本研究建立的人卵巢癌实体瘤和腹水瘤裸鼠移植模型与临床患者相似，为进一步研究人体卵巢癌发病和治疗提供了理想的实验动物模型。
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| 关 键 词： | 卵巢癌 裸鼠 移植瘤 腹水瘤 肿瘤移植 |
| 文章编号： | 1000-7431(2001)02-0082-03 |
| 修稿时间： | 1999-10-21 |
ESTABLISHMENT OFTRANSPLANTABLE AND ASCITES MODELS OF HUMAN OVARIAN CARCINOMA IN NUDE MICE AND THEIRMORPHOLOGICAL CHARACTERISTICS |
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| CHEN Ai ping,WANG He,PENG Zhi lan,et al.. ESTABLISHMENT OFTRANSPLANTABLE AND ASCITES MODELS OF HUMAN OVARIAN CARCINOMA IN NUDE MICE AND THEIRMORPHOLOGICAL CHARACTERISTICS[J]. Tumor, 2001, 21(2): 82-84 |
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| Authors: | CHEN Ai ping WANG He PENG Zhi lan et al. |
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| Affiliation: | CHEN Ai ping,WANG He,PENG Zhi lan,et al. Department of Obstetrics and Gynecology,The Second Affiliated Hospital,West China University of Medical Science |
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| Abstract: | Objective To establish a transplantable and ascites model of human ovarian carcinoma in nude mice. Methods Tumor tissues or ascites of human ovarian carcinoma were directly implanted subcutaneously or peritoneally into nude mice. Tumorgenicity, metastasis and morphological characteristics of transplanted tumors were studied by light and electron microscopy and chromosome analysis. Results 2 transplanted tumors and 1 ascite tumor were established and had been passaged in vivo for 6 and 18 generations respectively. Histological, electron microscopic observation, chromsomal pattern and tumor marker had been identified as the same characteristics as the human ovarian carcinoma and ascites. Conclusion The models provide useful tools for the study of genesis and treatment of human ovarian carcinoma. |
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| Keywords: | Ovarian neoplasms Mice nude Neoplasm transplantation Ascites tumor |
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