配位吸附树脂对沙棘中黄酮类成分的吸附分离

【目的】研究配位吸附树脂在非水体系中对沙棘中黄酮类成分的吸附情况。【方法】通过静态吸附与解吸实验考察树脂的吸附性能,选择对黄酮类成分吸附效果最佳的配位吸附树脂,对沙棘提取液中黄酮类成分进行分离纯化;采用HPLC法测定沙棘黄酮的含量。【结果】配位吸附树脂在环己烷体系中吸附作用较好,动态吸附饱和吸附量为33.16 mg/g;以5%HAc乙醇溶液为洗脱剂,消耗10BV溶剂洗脱率为93%;经过3次重复实验树脂吸附量及洗脱率基本没有变化。【结论】经配位吸附树脂吸附分离后沙棘提取物中黄酮纯度由14.93%提高到55.74%,起到纯化精制的目的。树脂不需要再生,连续使用吸附性能稳定。

Adsorption of flavonoids from Hippohae rhamnoides L. by coordination adsorption

Objective] To investigate the asdorption of flavonoids of Hippophae Rhamnoides L. by coordination resin in the non - aqueous system. Methods] Optimal conditions of adsorption and desurption were determined by studying the absorbability on the resinsPurifyiag the flavonoide from Hippophae Rhamnoides L extraction by the coordination resin, and asopting HPLC to measure the content of the flavonlids of Hippophae Rhamnoides L. Results] Quercetin was better adsorbed from n-hexane than other medias on the ligand exchange adsorbent. The maximum dynamic adsorption capacity was 33.16 mg/g, the eluting reagent was 5%HAC ethanol and spending 10BV the eluted ratio was 93%. The adsorbting and eluting efficiency of the hgand exchange ad- sorbent was stable and didn＇t change in three times applying. Conclusions] The 14.93% crude product of Hippohae rhamnoides L. is purified to 55.74 %. It shows that the coordination adsorption has higher absorption capacitity than the other resins and can be desorbed easily.