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| 芍药甙对大鼠空肠平滑肌细胞静息膜电位的作用研究 | |
| 引用本文： | 洪立立,朱敏,付炜,齐清会. 芍药甙对大鼠空肠平滑肌细胞静息膜电位的作用研究[J]. 武警医学院学报, 2005, 14(3): 189-191 |
| 作者姓名： | 洪立立 朱敏 付炜 齐清会 |
| 作者单位： | 1. 天津大港医院,天津,大港,300270 2. 天津市永久医院,天津,塘沽,300450 3. 大连医科大学第一附属医院,辽宁,大连,116011 |
| 摘 要： | 【目的】探讨芍药甙对大鼠空肠平滑肌细胞静息膜电位的调控作用。【方法】酶解法分离大鼠空肠环行平滑肌细胞,全细胞膜片钳方法测定膜电位。静息状态下,平滑肌细胞的膜电位为(-59.3±3.4)mV;加入芍药甙孵育后,膜电位降低至(-65.6±3.0)mV(P=0.007)。【结论】芍药甙可以通过开放Kv通道,使膜超极化,限制Ca2+内流,实现了对平滑肌细胞的舒张作用。 |
| 关 键 词： | 芍药甙 平滑肌细胞 膜电位 膜片钳 |
| 文章编号： | 1008-5041(2005)03-0189-03 |
| 修稿时间： | 2004-12-30 |
Regulating effects of Paeoniflorin on resting membrane potential in the circular smooth muscle cells of rat jejunum |
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| HONG Li-li,ZHU Min,FU Wei,QI Qing-hui. Regulating effects of Paeoniflorin on resting membrane potential in the circular smooth muscle cells of rat jejunum[J]. Acta Academiae Medicinae CPAPF, 2005, 14(3): 189-191 | |
| Authors: | HONG Li-li ZHU Min FU Wei QI Qing-hui |
| Abstract: | To determine the role of paeoniflorin in regulating resting membrane potential of smooth muscle cells in rat jejunum.Smooth muscle cells were isolated from the muscle layers of Wistar rat jejunum and membrane potential in single smooth muscle cell was measured using conventional whole cell patch-clamp technique.Extracellular application of paeoniflorin caused membrane potential hyperpolarization. [Conclusion]By regulating Kv channels, paeoniflorin induced Em hyperpolarization, decreased influx of calcium, and caused relaxation of smooth muscle cell. |
| Keywords: | Paeoniflorin Smooth muscle cell Membrane potential Patch clamp |
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