中药蒲黄提取液的镇痛作用研究

目的观察不同蒲黄提取液的镇痛作用，并考察蒲黄提取液的急性毒性反应。方法用水煎醇沉法、乙醇回流法等提取蒲黄的有效成分，以小鼠为实验对象，用热板法和扭体法测其镇痛作用。通过大剂量给药，观察小鼠毒性反应。结果蒲黄提取液对小鼠化学刺激和物理刺激致痛都有明显的镇痛作用，蒲黄醇提液的镇痛作用更加明显。急性毒性实验测不出蒲黄的LD50。结论蒲黄的乙醇提取液有显著的镇痛作用，大剂量未见明显小鼠急性毒性反应。

A Study of the Analgesic Effect and Acute Toxicity of the Extract of the Traditional Chinese Medicine Pollen Typhae

Objective To study the analgesic effect and acute toxicity of the extracts of Pollen typhae obtained with different extraction. Methods Active principles of Pollen typhae were extracted with the water decoction-alcohol sedimentation method and ethanol reflux method. Adult mice were used as the subjects of the experiment. The analgesic effect of the drug was examined with the hot-plate test and writhing reflex test while the acute toxicity was assayed by observing the effect of a single large dose of the ethanol extract of the drug administered by gastrogavage. Results Different extracts of Pollen typhae, in partieular the alcohol extract, were shown to exert remarkable analgesic effect on the sensation of pain caused by physical or chemical stimuli. No LD50 of the drug could be demonstrated. Conclusion The ethanol extract of Pollen typhae was shown to have excellent analgesic effect, and it should be safe to use the drug in the clinic with the suggested dose.