市售蜂蜜中污染微生物的调查分析

[目的]对市售54批次蜂蜜中的微生物污染情况进行调查分析.[方法]依据食品安全国家标准对蜂蜜中菌落总数、霉菌计数和嗜渗酵母计数进行检测和判定,通过形态学、基质辅助激光解吸电离飞行时间质谱、大亚基单元核糖体RNA基因序列对不合格样品中的细菌和真菌进行鉴定和分析.[结果]共3批次蜂蜜微生物超标,其中菌落总数超标1批次,检出...

Analysis of contaminated microorganisms in commercial honey

[Objective]To investigate the microbial contamination in 54 batches of commercial honey.[Methods]Aerobic plate colony counts for bacteria and colonies of mould and osmophilic yeasts in honey were determined according to the National Food Safety Standard. The bacteria and fungi in unqualified samples were further identified and analyzed by morphology,MALDI-TOF-MS and large subunit(LSU)rRNA gene sequence.[Results]Three unqualified batches were found. One batch had aerobic plate colony counts exceeding the standard,with a variety of bacteria including Bacillus sp.,Paenibacillus sp. and Brevibacillus sp. Two batches had mould counts exceeding the standard. Aspergillus sp was detected in both samples(from the same manufacturer),and the DNA sequence homology was very high,suggesting the mould might come from the same pollution source.[Conclusion]There are many kinds of microbial contamination in honey. Manufacturers should strengthen the microbe control in monitoring process to avoid the repeated microbial contamination.