| 中药脊髓Ⅰ号对脊髓厚片Ca~(2+)荧光强度的影响 |
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| 引用本文： | 高秀来,刘霞,马育平,陈亚亮,宋一志,景鹏,韩凤岳,苏静,刘卫东,张平,常超英,刘恩.中药脊髓Ⅰ号对脊髓厚片Ca~(2+)荧光强度的影响[J].解剖学研究,2004,26(1):32-34. |
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| 作者姓名： | 高秀来 刘霞 马育平 陈亚亮 宋一志 景鹏 韩凤岳 苏静 刘卫东 张平 常超英 刘恩 |
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| 作者单位： | 1. 首都医科大学解剖学教研室,北京,110001
2. 中国中医研究院骨伤科研究所 |
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| 基金项目： | 北京市教委1997年基金资助项目 |
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| 摘 要： | 目的 探讨中药脊髓Ⅰ号对脊髓厚片Ca2 + 荧光强度的影响。方法 将 33只Wistar大鼠随机分为 3组 :下胸段脊髓半横断组 (损伤组 )、对照组和中药脊髓Ⅰ号治疗组。快速处死大鼠 ,取下胸段脊髓 ,切脊髓厚片 ,用Fluo 3荧光染料孵育 ,激光共聚焦显微镜观察。结果 损伤组、对照组和中药治疗组左侧Ca2 + 荧光强度分别为 12 6 3± 3 2 7、13 34± 3 5 1和 12 89±3 31,右侧分别为 2 1 6 8± 3 6 9、14 72± 2 12和 12 97± 3 6 0 ,损伤组脊髓厚片损伤侧Ca2 + 荧光强度显著升高 ,治疗组两侧灰质Ca2 + 荧光强度无显著差异。结论 (1)损伤侧Ca2 + 荧光强度显著升高 ;(2 )中药脊髓Ⅰ号对Ca2 + 荧光强度的升高有一定抑制作用。
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| 关 键 词： | 脊髓 Ca2+荧光强度 大鼠 |
| 修稿时间： | 2003年7月26日 |
Effects of Chinese herb on the fluorescent intensity of the Ca2+ in gray matter in spinal cord |
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| Gao Xiulai,Liu Xia,Ma Yuping,Chen Yaliang,Song Yizhi,Jing Peng,Han Fengyue,Su Jing,Liu Weidong,Zhang Ping,Chang Chaoying,Liu En.Deparment of Anatomy,Capital University of Medical Sciences,Beijing China.Effects of Chinese herb on the fluorescent intensity of the Ca2+ in gray matter in spinal cord[J].Anatomy Research,2004,26(1):32-34. |
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| Authors: | Gao Xiulai Liu Xia Ma Yuping Chen Yaliang Song Yizhi Jing Peng Han Fengyue Su Jing Liu Weidong Zhang Ping Chang Chaoying Liu EnDeparment of Anatomy Capital University of Medical Sciences Beijing China |
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| Institution: | Gao Xiulai,Liu Xia,Ma Yuping,Chen Yaliang,Song Yizhi,Jing Peng,Han Fengyue,Su Jing,Liu Weidong,Zhang Ping,Chang Chaoying,Liu En.Deparment of Anatomy,Capital University of Medical Sciences,Beijing 110001 China |
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| Abstract: | Objective To study the effect of Chinese herb on the fluorescent intensity of the Ca 2+ in spinal cord after spinal cord hemisection.Methods The experimented rats were divided into three groups:the control group,the injured group and the group administered Chinese herb,the later two groups were operated spinal cord right-hemisection.We mensurated fluorescence intensity of the Ca 2+ in the spinal cord slice by laser scanning microscopy.Results The up-regulation of the fluorescence intensity of the Ca 2+ in injured side,and the fluorescent intensity of the Ca 2+ in the group administered Chinese herb was heightened not as much as in the injured group. Conclusions (1)the fluorescence intensity of the Ca 2+ was heightened after injury;(2)Chinese herb can help gray matter cells escape from increasing of Ca 2+,which benefit to cells survival. |
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| Keywords: | Spinal cord Fluorescence intensity of the Ca 2+ Rat |
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