治伤巴布剂对大鼠创伤性软组织肿胀模型骨骼肌中AQP-1表达的影响

目的:观察治伤巴布剂对大鼠急性软组织损伤模型骨骼肌中AQP-1表达的影响.方法:SD大鼠54只,随机分成3组,分别为正常对照组、模型组和药物处理组.造模后1h、6h、1d、3d、5d、7d6个时相点处死动物,每组每个时相点3只,在标记的区域内肌肉组织取材,采用干湿比重法测定骨骼肌组织的含水量,应用Western Blot、qPCR法检测AQP-1蛋白及基因的表达水平,并进行相关分析.结果:检测的6个时相点中,模型组和药物处理组的肌肉组织含水量均高于正常对照组,在3d、5d、7d3个时相点,药物处理组的含水量低于模型组,药物处理组、模型组的AQP-1蛋白及基因表达水平均高于正常对照组,而药物处理组与模型组相比,差异具有统计学意义.结论:治伤巴布剂具有减轻急性软组织肿胀的作用,从而加速急性软组织损伤后修复的进程.

Effect of Curing-injury Cataplasma on Expression of AQP-1 in Skeletal Muscles of Rat Model with Acute Injury in Soft Tissues

This study was aimed to observe the effect of curing-injury cataplasma on the expression of AQP-1 in skeletal muscles of rat model with acute injury in soft tissues. A total of 54 SD rats were randomly divided into three groups. In the normal control group, no attack was given in the modeling. In the model group, on the depilatory area, blowing apparatus was used to attack marked range for the establishment of the model with soft tissue swelling with acute injury. In the drug treatment group, curing-injury cataplasma was scattered on the stricken area immediately after the model establishment of soft tissue swelling with acute injury and fixed with bandage. After modeling, rats were killed respectively in six time phase points, namely 1 h, 6 h, 1 d, 3 d, 5 d, and 7 d, three in each group at each point. In marked area some tissues were removed. In the taken tissues, wet-to-dry specific gravity method was used for the detection of the water content of skeletal muscle. Western blotting and qPCR method were used for the detection of AQP-1 protein and the level of gene expression. Then, correlation analysis was made. The results showed that in all six time phase points, in the modeling and drug treatment groups, the water content of skeletal muscle was higher than that of the normal control group. In three phase points of 3 d, 5 d and 7 d, the water content in the drug treatment group was lower than that of the modeling group. And for the modeling and drug treatment groups, the AQP-1 protein and the level of gene expression were higher than that of normal control group. There was a statistically significant difference between drug treatment and model group. It was concluded that the curing-injury cataplasma can relieve soft tissue swelling with acute injury, and accelerate the process of repair after the injury.