| ehCGβ肿瘤基因疫苗诱生的效应脾细胞过继免疫抗肿瘤作用的研究 |
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| 引用本文： | 关庆东,王立新,张进平,徐 薇,储以微,王 缨,熊思东.ehCGβ肿瘤基因疫苗诱生的效应脾细胞过继免疫抗肿瘤作用的研究[J].中国肿瘤生物治疗杂志,2004,11(2):79-83. |
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| 作者姓名： | 关庆东 王立新 张进平 徐 薇 储以微 王 缨 熊思东 |
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| 作者单位： | 复旦大学上海医学院免疫学系教育部分子医学重点实验室,上海基因免疫与疫苗研究中心,上海,200032 |
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| 基金项目： | 上海市科技攻关项目，国家自然科学基金 |
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| 摘 要： | 目的:探讨ehCGβ肿瘤基因疫苗诱生的效应脾细胞过继免疫在抗肿瘤中的作用.方法:通过转染建立稳定表达ehCGβ和HBV-preS2/S的细胞株Sp2/0-ehCGβ和Sp2/0-preS2/S.以TR421-hCGβ质粒实施基因免疫,免疫后检测小鼠脾细胞,特异性细胞毒活性;同期将脾细胞过继免疫给正常小鼠,以过继TR421-hCGβ质粒免疫小鼠脾细胞为实验组、过继TR421质粒免疫小鼠脾细胞为对照组,检测脾细胞杀伤效应.结果:效应脾细胞对SP2/0-ehCGβ的杀伤率明显高于Sp2/0-preS2/S(P＜0.05).Sp2/0-ehCGβ在实验组小鼠仅2只形成实体瘤,TR421-hcGβ质粒基因免疫抗肿瘤任用有肿瘤特异性,两组有显著性差异(P＜0.05),而在对照组小鼠均形成实体瘤.Sp2/0-preS2/S在所有的小鼠均形成了实体瘤,其瘤重无显著性差异.结论:ehCGβ肿瘤基因疫苗诱生的效应细胞可特异性杀伤肿瘤,过继免疫效应细胞能使正常小鼠获得明显的特异性抗肿瘤能力.
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| 关 键 词： | 异位人绒毛膜促性腺激素 效应脾细胞 肿瘤 基因疫苗 过继免疫 |
| 文章编号： | 1007-385X(2004)02-0079-05 |
| 收稿时间： | 2003/11/27 0:00:00 |
| 修稿时间： | 2003年11月27 |
Anti-Tumor Effects of Adoptively Transferred Splenocytes Induced by ehCGβ Gene Immunization |
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| GUAN Qing dong,WANG Li xing,ZHANG Jin-ping,XU Wei,CHU Yi-wei,WANG Ying and XIONG Si-dong.Anti-Tumor Effects of Adoptively Transferred Splenocytes Induced by ehCGβ Gene Immunization[J].Chinese Journal of Cancer Biotherapy,2004,11(2):79-83. |
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| Authors: | GUAN Qing dong WANG Li xing ZHANG Jin-ping XU Wei CHU Yi-wei WANG Ying and XIONG Si-dong |
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| Institution: | Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University; Center for Gene Immunization and Vaccine Research, Shanghai 200032, China;Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University; Center for Gene Immunization and Vaccine Research, Shanghai 200032, China;Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University; Center for Gene Immunization and Vaccine Research, Shanghai 200032, China;Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University; Center for Gene Immunization and Vaccine Research, Shanghai 200032, China;Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University; Center for Gene Immunization and Vaccine Research, Shanghai 200032, China;Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University; Center for Gene Immunization and Vaccine Research, Shanghai 200032, China;Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University; Center for Gene Immunization and Vaccine Research, Shanghai 200032, China |
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| Abstract: | |
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| Keywords: | ectopic human chorionic gonadotrophin(ehCG) effect splenocytes tumor gene vaccine adoptive immunization |
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