生长相关性癌基因-1在喉癌中的表达及其与VEGF的相关性

目的：探讨生长相关性癌基因-1（GRO-1）在喉癌组织中的表达及其与喉癌部分临床生物学特性的相关性。方法：采用RT-PCR方法检测GRO-1在30例喉鳞状细胞癌组织（喉癌组）和10例正常喉黏膜组织（对照组）中的表达；免疫组织化学染色方法检测60例喉癌组织中GRO-1和血管内皮生长因子（VEGF）的表达。结果：RT-PCR结果显示，GRO-1在喉癌组中的表达与对照组相比差异有统计学意义（P〈0.01）。免疫组织化学染色结果显示，GRO-1在有淋巴结转移的喉癌中的表达相对无淋巴结转移者差异有统计学意义（P〈0．05）；GRO-1表达与喉癌分化程度无关（P〉0.05）；GRO-1在不同年龄、性别、病变部位和不同T分期中的表达均无统计学意义（均P〉0.05）。GRO-1和VEGF的表达存在正相关关系（P〈0.05）。结论：GRO-1能够通过多种作用影响喉癌的发生、发展和转移，其中，在微血管形成方面与VEGF的高表达紧密关联。

Expression of GRO-1 and its relationship with VEGF in squamous cell carcinoma of larynx

OBJECTIVE: To investigate the correlation between expression of growth regulated oncongene 1 (GRO-1) and some clinical biological characteristies of laryngeal carcinoma. METHOD: Expression of GRO-1 and VEGF(vascular endothelial growth factor) protein were investigated by means of immunohistochemistry in 60 laryngeal carcinoma tissues, GRO-1 was detected by RT-PCR in 30 laryngeal carcinoma tissues and 10 normal laryngeal tissues. RESULT: The result of RT-PCR showed that the expression of GRO-1 in squamous cell carcinoma of larynx tissues was higher than those in normal laryngeal tissues (P < 0.01). The result of Immunohistochemistry revealed that the expression of GRO-1 in squamous cell carcinoma of larynx with the metastasis of lymph nodes was higher than those without metastasis (P < 0.05). There was no significant relationship between GRO-1 protein expression and patients'tumor histological difference (P > 0.05). The expression of GRO-1 had no significant difference in age, sex, location and T classification (P > 0.05). There was positive correlation in staining intensity beween GRO-1 and VEGF (P < 0.05). CONCLUSION: GRO-1 can promote tumor growth, metastasis by several mechanism,the effect of GRO-1 on angiogensis are consistent with the distribution of VEGF.