液氮冻存人同种心脏瓣膜和大动脉免疫原性的对比

目的进行液氮冻存人同种带瓣主、肺动脉(CVH)瓣叶、动脉壁和瓣下心肌组织的免疫原性对比研究。方法采用CVH瓣叶、动脉壁的单细胞悬液分别与相同血型受体的淋巴细胞混合培养法,检测混合培养后不同时点CD25、HLA-DR的表达;并与单纯淋巴细胞培养组(对照组)进行对比研究。结果CVH动脉壁的免疫原性较其瓣叶强,表现为CD25、HLA-DR高水平表达;未能获得CVH瓣下心肌的细胞悬液。结论液氮冻存人CVH动脉壁的免疫原性较其瓣叶的免疫原性强。

Comparative study on the immunogenicity of human homologous cardiac valve and great artery homograft cryopreserved with liquid nitrogen

Objective To study the difference of the immunogenicity of the leaflets,arterial wall and myocardium of conduit valved homograft(CVH)cryopreserved with liquid nitrogen.Methods Mono-cell suspension of leaflets cells and arterial cells of CVH were co-cultured with human lymphatic cells.Expressive level of CD25 and HLA-DR of these lymphatic cells was detected by flow cytometry in the different cultural duration and compared with that of lymph cells alone cultured(comparative group).Results The immunogenicity of CVH artery walls was higher than that of CVH leaflets,and expressive level of whose CD25 and HLA-DR was higher too.The immunogenicity of CVH myocardium was not studied because the myocardial cell suspension was not available.Conclusion The immunogenicity of arterial walls of cryopreserved CVH is higher than that of leaflets.