熊果酸抑制人胃腺癌SGC-7901细胞系COX-2的表达

目的观察熊果酸(UA)对胃腺癌细胞SGC-7901环氧化酶-2(COX-2)表达的影响。方法采用MTT法观察细胞增殖;Western blot法检测COX-2蛋白表达;放射免疫分析法观察细胞合成PGE2。结果UA可以抑制SGC-7901细胞的增殖,并呈浓度和时间依赖性;外源性PGE2并不能逆转UA的抑制增殖作用;UA可以降低细胞COX-2蛋白的表达,并呈浓度依赖性;UA可以抑制PGE2的合成,但该作用无浓度依赖性。结论UA通过抑制COX-2的表达、减少PGE2的合成抑制胃腺癌细胞SGC-7901的增殖,可能成为一种胃癌预防剂。

Inhibition of ursolic acid on COX-2 expression of human gastric cancer cells in vitro

Objective To investigate the inhibitory role of ursolic acid on COX-2 expression of human gastric cancer cells SGC-7901. Methods The anti-proliferation effect of ursolic acid on SGC-7901 and effect of exogenous prostaglandins E2 on cell proliferation were determined by MTT assay; Western blot assay was used to identify expression of COX-2 protein; The effect of ursolic acid on PGE2 production in SGC-7901 cells were examined by radioimmunoassay(RIA). Results Ursolic acid inhibited the proliferation of SGC-7901 cells in a time-response and concentration-response manner; This effect was irreversible in the presence of exogenously added prostaglandin E2; COX-2 protein level was decreased by ursolic acid and then PGE2 production was inhibited. Conclusion Ursolic acid decreases the proliferation of SGC-7901 by inhibits COX-2 protein and PGE2 expression which suggest a possible mechanism for its chemopreventive and anticancer effects.