| 神经生长因子对人视网膜血管内皮细胞的影响 |
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| 引用本文： | 王丁丁,于强,陈子林,柳夏林.神经生长因子对人视网膜血管内皮细胞的影响[J].中山大学学报(医学科学版),2010,31(1):45-49. |
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| 作者姓名： | 王丁丁 于强 陈子林 柳夏林 |
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| 作者单位： | 1. 惠州市中心人民医院眼科, 广东 惠州 516001; 2. 眼科学国家重点实验室∥中山大学中山眼科中心, 广东 广州 510060 |
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| 摘 要： | 【目的】 观察神经生长因子(NGF)及TrKa阻断剂K252a对体外培养的人视网膜血管内皮细胞(HRCEC)增殖的影响｡ 【方法】 MTT法检测不同因子(NGF各浓度组､NGF + K252a各浓度组､bFGF组､bFGF + K252a组和正常培养液组)对正常和缺氧条件下培养的HRCEC的影响｡ 【结果】 随NGF浓度增加(20､50､100 ng/ml),HRCEC细胞数目明显增多(正常氧浓度NGF组分别为0.254 ± 0.033､0.696 ± 0.029､1.136 ± 0.051;缺氧条件相应各组为0.422 ± 0.036､0.798 ± 0.044､1.376 ± 0.052,P均 < 0.05)｡随K252a浓度增加(50､100､200 nmol/L),NGF + K252a组正常氧浓度时分别为0.864 ± 0.067､0.496 ± 0.025､0.202 ± 0.078,缺氧条件时相应为1.042 ± 0.047､0.700 ± 0.065､0.401 ± 0.078,较同浓度NGF(100 ng/ml)组HRCEC细胞数目减少(P < 0.05),随K252a浓度增加HRCEC细胞数目减少趋势愈加显著(P均 < 0.05)｡【结论】 NGF可促进HRCEC的增殖,该作用可被特异性TrkA阻断剂K252a所阻断｡
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| 关 键 词： | 神经生长因子(NGF) 人视网膜血管内皮细胞(HRCEC) TrkA阻断剂K252a |
| 收稿时间： | 2009-08-15; |
Effect of NGF on Cultured Human Retinal Capillary Endothelial Cells (HRCEC) |
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| WANG Ding-ding,YU Qiang,CHEN Zi-lin,LIU Xia-lin.Effect of NGF on Cultured Human Retinal Capillary Endothelial Cells (HRCEC)[J].Journal of Sun Yatsen University(Medical Sciences),2010,31(1):45-49. |
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| Authors: | WANG Ding-ding YU Qiang CHEN Zi-lin LIU Xia-lin |
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| Institution: | WANG Ding-ding1,YU Qiang2,CHEN Zi-lin1,LIU Xia-lin2 (1.Department of Ophthalmology,Huizhou Central People\'s Hospital,Huizhou 516001,China,2.State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China) |
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| Abstract: | Objective] To observe NGF on cultured human retinal vascular endothelial cells (HRCEC) proliferation. Methods] The MTT assay was used to analyze the impact of culture HRCEC on different factors (NGF concentration groups, NGF + K252a concentration groups, bFGF group, bFGF + K252a groups, the normal culture medium groups) in normal and hypoxic condition. Results] With the increase of NGF concentration (20,50,100 ng/mL), HRCEC significantly increased (normal condition: 0.254±0.033,0.696±0.029, 1.136±0.051; hypoxic condition: 0.422±0.036, 0.798±0.044, 1.376±0.052, P< 0.05). Compared NGF + K252a group with the same concentration of NGF (100 ng/ml) group, HRCEC reduced (P<0.05), with increasing the concentration of K252a (50,100,200 nmol/L), the trend of HRCEC decreasing is become more significant (normal condition:0.864±0.067, 0.496±0.025, 0.202±0.078; hypoxic condition:K252a 1.042±0.047,0.700±0.065, 0.401±0.078, P<0.05). Conclusion] NGF can promote the proliferation of HRCEC, the effect could be specifically blocked by TrkA inhibitor K252a. |
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| Keywords: | nerve growth factor (NGF) human retinal vascular endothelial cells (HRCEC) TrkA inhibitor (K252a) |
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