川芎嗪对顺铂所致大鼠肾氧化损伤的保护作用

目的探讨川芎嗪（TMP）对顺铂（DDP）所致大鼠肾氧化损伤影响。方法将32只SD大鼠随机分成4组各8只：DDP+TMP 50 mg•kg－1•d－1组和DDP+TMP 100 mg•kg－1•d－1组分别腹腔注射TMP 50和100 mg•kg－1•d－1，连续5 d；对照组和DDP组给予0.9%氯化钠溶液，均5 mL•kg－1。给药第3天，除对照组外，其他3组腹腔注射DDP，8 mg•kg－1。实验第5天收集代谢笼中大鼠24 h尿，测尿蛋白含量；处死大鼠后测血清尿素氮（BUN）、肌酐（SCr）、24 h尿蛋白、肾皮质丙二醛（MDA）、还原型谷胱甘肽（GSH）、过氧化物歧化酶（SOD）、谷胱甘肽 S 转移酶（GST）活性及肾皮质一氧化氮（NO）含量、一氧化氮合酶（NOS）活性。结果与DDP组比较，DDP+TMP50 mg•kg－1•d－1组和DDP+TMP100 mg•kg－1•d－1组大鼠24 h尿蛋白分别下降41.45%和65.33%；BUN分别下降18.12%和57.51%；SCr分别下降14.39%和48.89%（P＜0.05或P＜0.01）；与DDP组比较，DDP+TMP 100 mg•kg－1•d－1组大鼠肾皮质MDA、NO含量及NOS活力分别降低36.73%，45.39%，22.86%（P＜0.05），GSH含量和SOD、GST活性分别为DDP组的1.33，1.66，1.57倍（P＜0.05）。结论TMP对DDP所致大鼠肾氧化性损伤具有保护作用。

Protective Effects of Ligustrazine on Cisplatin-Induced Oxidative Injury of Kidneys in Rats

Objective To investigate protective effects of ligustrazine(tetramethypyrazine,TMP) on cisplatin-induced oxidative injury of kidneys in rats.Methods TMP was administered at 50,100 mg·kg-1·d-1 body weight intraperitoneally(ip) for five consecutive days,2 days before a single dose of cisplatin(DDP,8 mg·kg-1,ip).The rats were sacrificed four hours after the last injection.Blood urea nitrogen(BUN) and serum creatinine(SCr) were tested,24 hours urine were collected to detect urinary protein.The contents of malondialdehyde(MDA),glutathione(GSH) content,nitric oxide(NO),activities of superoxide dismutase(SOD),glutathione-S-transferase(GST),the nitric oxide synthase(NOS) in the renal cortex were measured.Results Compared with DDP,TMP(50 and 100 mg·kg-1·d-1) dose-dependently reduced 24 h urinary protein,BUN and SCr induced by cisplatin,by 41.45%,65.33%;18.12%,57.51%;14.39%,48.89%(P<0.05,P<0.01),respectively.The 100 mg·kg-1·d-1 TMP significantly decreased levels of MDA,NO,NOS and GSH,SOD,GST induced by cisplatin in kidney by 36.73%,45.39%,22.86%(P<0.05),respectively;while,the content of GSH was increased by 1.33-fold,1.66-fold,1.57-fold(P<0.05),respectively,compared with that in the DDP group.Conclusion TMP could protect cisplatin-induced oxidative nephrotoxicity.