免疫相关基因在同一膀胱癌亚克隆细胞株中的差异表达

目的:筛选和鉴定同一膀胱癌亚克隆细胞株中差异表达的与BCG免疫相关的基因。方法:以已建立的两株同一来源、不同生物学表型的膀胱移行细胞癌细胞系BLX和BLS211为材料,采用抑制性削减杂交技术(SSH)筛选差异表达的基因片段。结果:在BLX细胞中获得9个高表达的基因,在BLS211细胞中获得15个高表达的基因。BLX细胞中有3个基因,即与卡介苗(BCG)免疫治疗相关的基因BCG诱导的膜蛋白(BIGM103)基因、纤维连接蛋白(FN)基因和补体因子B(BF)基因;BLS211细胞中有8个基因均为新的表达序列标签(EST),已被GenBank登录(登录号为DY50570813,DY2304478)。结论:SSH技术筛选表明,BIGM103等免疫相关基因的不同,可能与不同膀胱癌细胞对BCG免疫治疗的差异性有关;新的EST的获得,为进一步克隆全长、研究基因的功能创造了条件。

Differential expression of immune-associated genes in two subcloned cell lines from a same human bladder cancer

AIM: To screen and identify differentially expressed genes in subcloned lines from a same human bladder transitional cell carcinoma (TCC). METHODS: Two bladder TCC cell lines (BLX and BLS-211) with different phenotypes but same origin were used to screen for differentially expressed genes by suppression subtractive hybridization (SSH). RESULTS: 9 over-expressed genes in BLX and 15 in BLS-211 cells were obtained, respectively. Some Bacillus Galmette-Guerin(BCG)- associated genes, such as BCG induced integral membrane protein(BIGM103), fibronectin(FN), complement factor B(BF), were over-expressed in BLX cells. And 8 new ESTs(Expressed Sequence Tag) in BLS-211 cells were collected by GenBank dbEST database with the accession number of DY505708-13, DY230447-8. CONCLUSION: SSH is a powerful method for the identification of differentially expressed genes in different cell lines or clones. Some BCG-associated genes, which are differentially expressed in different cells may contribute to the different response to clinical BCG therapy. The identified new ESTs can be cloned for full length to further study their functions.