P53、P16、Bcl-2基因及产物在原发性肺癌中的表达及其临床病理意义

目的 :探讨原发性肺癌 Mtp5 3、p16、Bcl- 2的异常表达与肺癌发生、发展的关系 ,以及它们之间的调控关系。 方法 :用免疫组化技术检测 (L SAB) 114例原发性肺癌组织中 p5 3、p16、Bcl- 2的表达。并用 PCR技术对 6 2例 p16蛋白丢失的肺癌组织中 p16外显子 2 (p16 E2 )的缺失进行分析。 结果:p5 3蛋白异常表达与肺癌组织学类型、分化程度无关 (P >0 .0 5 ) ,但与淋巴结转移情况有关 (P >0 .0 5 )。 p16蛋白阳性表达率与肺癌的组织学类型无关 ,但是其表达水平与非小细胞肺癌 (NSCL C)的细胞分化程度和淋巴结转移密切相关 (P <0 .0 5 )。 NSCL C中 p16E2的缺失率为 45 .2 %,其缺失水平随淋巴结转移和组织学分级的升高而升高 (P <0 .0 5 )。 SCL C(小细胞肺癌 )和正常肺组织中无 p16 E2的缺失。 Bcl- 2在小细胞肺癌中阳性率 6 2 .2 %显著高于 Sq Ca(鳞癌 )的 2 5 %和 Ad Ca(腺癌 )的 9.1%(P <0 .0 5 ) ,与细胞分化程度和淋巴结转移情况无关。另外 ,p5 3与 p16蛋白之间存在调控关系。 结论 :(1) p16、p5 3、Bcl- 2的异常参与肺癌的发生、发展过程。 (2 ) p16基因及产物的异常表达与 NSCL C的组织学分级和淋巴结转移相关 ,可能参与 NSCL C的发生、发展和转移过程。 (3) Bcl- 2反映 SCL C的分之生物学行为和临床

The clinicopathological sigificance of expression of mtp53, Bcl-2, p16 gene and protein in primary lung cancer

Objective: To explore the relationship between abnormal expression of mtp53, p16 and Bcl-2 protein and oncogenesis and development of lung cancer. Methods: Expression of mtp53, p16 and Bcl-2 protein was detected in 114 lung cancer tissues by immunohistochemistry. Loss of p16 INK4 gene exon 2(E2) was analyzed using PCR technique in 62 cancer samples with p16 protein-negative stain and 14 paracancerous lung tissues. Results: Overexpression of mtp53 protein was not related to pathological type and histological grade of lung cancer (P>0.05), but correlated with lymph node status (P<0.05). Postive expression of p16 was closely related to histological grade of lung cancer and lymph node metastasis (P<0.05), but not to pathological type (P>0.05). The deletion of p16 E2 was 45.2% in nsclc and closely related to lymph node metastasis and histological grade (P<0.05). The postive rate of bcl-2 protein expression in sclc was significantly higher than that in nsclc (P<0.05). Besides, there is regulation between p53 and p16. Conclusions: (1) mtp53,p16 and Bcl-2 genes may play important roles in the oncogenesis and development of lung cancer. (2) It exists negative regulation between mtp53 and p16 protein.