PCR-ELISA微孔板杂交技术检测结核杆菌DNA

目的　采用PCR ELISA微孔板杂交技术检测结核杆菌DNA。方法 采用PCR技术扩增结核杆菌DNA，并将扩增产物加入预先包被结核杆菌探针的微孔板，再加入结核杆菌显色探针，同时进行微孔板核酸夹心杂交ELISA显色。共检测肺部疾病患者痰标本 510例。结果 该法的灵敏度为 59.3%，特异性为 95.0%；阳性预测值为 96.3%，阴性预测值为 51.4%。结论 PCR ELISA微孔板杂交技术可快速、准确地检测结核杆菌DNA，是结核病早期诊断和鉴别诊断的可靠实验室方法。

PCR microplate hybridization-ELISA for identification of tubercle bacillus DNA

Objective To identify the tubercle bacillus DNA with PCR microplate hybridization-ELISA. Methods To use PCR technology to expand tubercle bacillus DNA,and add the expanded matter into microplate with tubercle bacillus probes,then add tubercle bacillus probes of color displaying,and conduct color displaying of ELISA of microplate hybirdizationg.510 patients with pulmonary disease were tested.Results The sensitivity of the method is 59.3%,and its specificity is 95.0%.Positive predictive value is 96.3%,negative predictive value is 51.4%.Conclusion PCR microplate hybirdizatin-ELISA can be used to test tubercle bacillus DNA quickly and accurately,it is a reliable laboratory method early to diagnose and differentiate tuberculosis.