光敏生物素标记质粒DNA检测双链DNA抗体ELISA的建立及初步应用

建立一种既可定性也可定量检测血清抗双链ＤＮＡ抗体的ＥＬＩＳＡ，进一步提高对系统性红斑狼疮（ＳＬＥ）诊断和监测的水平。以链霉亲合素处理微孔板，包被光敏生物素标记的质粒ＤＮＡ，用ＨＲＰ－羊抗人ＩｇＧ测定血不表双链ＤＮＡ抗体。分别以自制ＥＬＩＳＡ和Ｂｉｎｄｉｎｇ ｓｉｔｅ公司ＥＬＩＳＡ抗ｄｓＤＮＡ定量试剂盒和胶体金斑点免疫渗滤法定性检测了３２例活动期ＳＬＥ，６例类风湿关节炎（ＲＡ），３０例健康人血清，阳

Estabilishment and initial application of photobiotinylated plasmid DNA ELISA for the detection of anti dsDNA

To estabilish a qualitative as well as quantitative ELISA for the detection of anti dsDNA to improve diagnostic and monitoring level for SLE.Photobiotinylated plasmid ds DNA is coated after streptavidin procoated,to detect serum anti dsDNA with HRP sheep anti human IgG.Using a self prepared coated ELISA plate and anti dsDNA quantitative kit of Binding site company of Birmingham medical college U K,and dot immunogold filtration assay have detedted sera of 32 patients with active SLE,6 rheumatoid arthritis and 30 normal controls,results showed,positive consistent rate is respectively 96 9% and 93 8% and negative consistent rate is 96 7%,5 patients with SLE were tested with the detection of quantitative,results were almost consistent.Initial application indicated the ELISA plate achieved good results and worth further studying and expanding detectable serum samples of patients.