食管鳞癌组织中肺耐药蛋白表达和DNA含量的定量分析

目的：探讨食管鳞癌组织中肺耐药相关蛋白（lung resistance-related protein,LRP）的表达和DNA含量检测的临床意义。方法：应用流式细胞术（flow cytometry,FCM）定量分析52例原发食管鳞癌组织和相应癌旁组织中LRP蛋白表达及DNA含量的变化状况。结果：食管鳞癌组织LRP表达的相对荧光强度（RFI）的中位数（M）为1.39,而相应癌旁组织为0.75;差异有统计学意义,P〈0.05。癌组织DNA指数（DI）、SPF和PI显著高于相应癌旁组织,P〈0.05。LRP蛋白表达在不同的性别、病理分级、临床分期和有无淋巴结转移间的表达均差异无统计学意义,P〉0.05。DNA倍体和SPF、PI均与性别、病理分级和临床分期无明显关系,P〉0.05;但与有无淋巴结转移有关,P〈0.05。非整倍体肿瘤患者淋巴结转移率（63.9%,23/36）高于二倍体肿瘤患者淋巴结转移率（18.8%,3/16）,P〈0.05。有淋巴结转移患者SPF和PI显著高于无淋巴结转移患者,P〈0.05。非整倍体肿瘤LRP蛋白表达水平（Median=1.42）略高于二倍体肿瘤（Median=1.35）,但两者差异无统计学意义,P〉0.05。结论：LRP蛋白表达和DNA含量在食管鳞癌发生中起重要作用。DNA含量与淋巴结转移有关,可作为预测食管鳞癌预后的指标。

Quantitative analysis of lung resistance protein expression and DNA content in esophageal squamous cell carcinoma patients

OBJECTIVE:To explore the clinical significance of lung resistance protein (LRP) expression and DNA content in esophageal squamous cell carcinoma patients. METHODS: Flow cytometry was used to analyze the LRP expression level and DNA ploidy in cancer tissues, corresponding para-cancer tissues of 52 primary esophageal squamous cell carcinoma patients. RESULTS: The relative fluorescence intensity (RFI) expressed by median (M) of LRP expression in the esophageal squamous carcinoma tissues was 1.39, and in the corresponding para-carcinoma tissues, was 0.75. There was a significant difference of LRP expression between the esophageal squamous carcinoma tissues and corresponding para-carcinoma tissues,P<0.05. DNA index (DI) and s-phase fraction (SPF) and proliferation index (PI) were higher in the esophageal squamous cell carcinoma tissues than those in the para-carcinoma tissues,P<0.05. There were no significant differences of LRP protein expressions in different gender, different pathological stages, different clinical stages, lymph node metastasis positive and negative patients, P>0.05. DNA ploidy, SPF and PI had no significant relations with gender, pathological stages, clinical stages (P<0.05), but had significant relations with lymph node metastasis. Lymph node metastasis positive rate in 36 noneupliody patients (63.9%,23/36) was higher than those in 16 diploidy patients(18.8%,3/16),P<0.05. SPF and PI in lymph node metastasis positive patients were higher than those in lymph node metastasis negative patients,P<0.05. The expression level of LRP in noneuploidy tumors (M=1.42) was higher than that in diploidy tumors (M=1.35), but there was no statistic difference, P>0.05. CONCLUSIONS: LRP protein expression and DNA content play an important role in tumor genesis of esophageal squamous cell carcinoma. DNA content is related to lymph node metastasis, and it could become the parameter in predicting the prognosis of esophageal cell carcinoma patients.