CFTR在超排卵幼龄大鼠卵巢的分布与表达

目的探讨囊性纤维化跨膜转导调节器(CFTR)在超排卵幼龄大鼠各期卵泡和黄体中表达的变化及其意义。方法采用免疫组化和免疫印迹方法检测超排卵幼龄Wistar大鼠注射HCG前,注射HCG后24h、48h及72h卵巢中CFTR的蛋白分布与含量变化。结果CFTR在超排卵幼龄Wistar大鼠的各级卵泡颗粒细胞、黄体细胞均有表达,随着黄体期延长其表达量逐渐下降,各时段卵巢表达量比较有显著性差异。免疫印记法证实各时段卵巢中均存在特异性的、相对分子质量为170KD的CFTR条带。结论卵泡期CFTR表达可能促进卵泡中卵泡液的积聚,卵泡直径增大,利于卵泡成熟;而黄体期CFTR表达下降则可能利于黄体形成,CFTR过多表达可能导致卵泡囊肿和黄体囊肿。

Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Rat Ovary

Objective To investigated the expression of cystic fibrosis transmembrane conductance regulator(CFTR),a cAMP-activated Cl-channel,in ovarian stimulated premature female rat ovary during a cycle of follicle development and corpus luteum formation at protein level.Methods Animals were injected with 10 IU of PMSG and were given 10 IU of HCG 48h later.Time-course experiments were performed at 0h before,and 24,48,72h after HCG treatment.Results Immunohistochemistry showed that administration of PMSG stimulated CFTR expression in thecal cell layer and granulosa cell layer of mature follicles 48h post injection,coincident with the PMSG-induced peak in follicular estradiol.However,the expression of CFTR was time-course reduced followed by HCG injection,in accordance with the gradually increased Plevel during luteum corpus formation.Western blotting analysis demonstrated that rat ovarian tissue expressed the special CFTR band at 170 KD.Conclusion cAMP-dependent Cl-channels are involved in regulation of follicle development and luteum formation.