鬼臼酰肼对白血病K562细胞株作用的实验研究

目的:研究鬼臼毒素衍生物鬼臼酰肼对K562白血病细胞的抑制增殖、诱导细胞凋亡作用.方法:采用集落形成实验、MTT比色法观察鬼臼酰肼对K562白血病细胞增殖的影响;流式细胞仪等技术手段研究鬼臼酰肼对K562细胞凋亡的影响.结果:鬼臼酰肼能抑制K562白血病细胞增殖,与对照组相比有显著性差异(P＜0.01),抑制作用与剂量呈显著性正相关.鬼臼酰肼可诱导K562细胞的凋亡,在5.0 μg/ml处理48 h,鬼臼酰肼组凋亡百分率33.5%.结论:鬼臼酰肼具有抗肿瘤活性,鬼臼毒素衍生物的不同结构对抗肿瘤活性有一定影响.

An Experimental Study on Proliferative Effects and Apoptotic Effects of Podophyllic Acid in K562 Cells

OBJECTIVE: To study the effects of podophyllic acid on proliferation and apoptosis in human leukemia K562 cells. METHODS: The inhibitory effects of podophyllic acid on K562 cells were investigated by clony-formation and MTT colorimetric assay. The apoptotic effects were observed by FCM. RESULTS: Podophylic acid could inhibit the proliferation of K562 cells and there were a significant difference between experimental and control groups (P < 0.01). There was a marked positive correlation between drug concentration and the inhibitory rates. Podophyllic acid could induce apoptosis of K562 cells. When K562 cells were treated with 5.0 microg/ml podophyllic acid for 48 hours, apoptosis occurred to the largest extent. The apoptosis rate of podophyllic acid was 33.5%. CONCLUSIONS: Podophyllic acid has antitumor effect, but the change of structure affects the function of derivatives of podophylloxin greatly.