| 肺炎克雷伯菌多药耐药株的耐药机制研究 |
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| 引用本文： | 朱健铭,孙晓珍,姜如金,吴康乐,王建敏,马兆龙. 肺炎克雷伯菌多药耐药株的耐药机制研究[J]. 中华医院感染学杂志, 2010, 20(17) |
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| 作者姓名： | 朱健铭 孙晓珍 姜如金 吴康乐 王建敏 马兆龙 |
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| 作者单位： | 杭州市余杭区中医院检验科,浙江,杭州,311106 |
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| 摘 要： | 目的 调查1株多药耐药肺炎克雷伯菌(MDRKP)58种耐药相关基因与可移动遗传元件的存在情况. 方法 对分离自我国内地的1株MDRKP,采用聚合酶链反应(PCR)及序列分析的方法分析58种β-内酰胺类、氨基糖苷类,喹诺刚类和其他广谱抗菌药物耐药相关基因与可移动遗传元件. 结果 该株MDRKP检出TEM-1、SHV-11(均测序比对证实)、tetA、mafA、trbC、tnp513、ISEcp1基因,且gyrA基因存在突变,其余50种基因未检出. 结论 该菌株耐Ⅰ～Ⅲ代头孢菌素抗菌药物,可能与同时产TEM-1型和SHV-11型β-内酰胺酶引起牵制耐药机制有关,该株MDRKP表型为多药耐药与gyrA基因突变及获得可移动遗传元件和多种耐药基因相关,在肺炎克雷伯菌中检出ISEcp1为国内首次报道.
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| 关 键 词： | 肺炎克雷伯菌 耐药基因 多药耐药 牵制耐药机制 |
Resistant Mechanisms of Multi-drug Resistant Klebsiella pneumoniae |
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| ZHU Jian-ming,SUN Xiao-zhen,JIANG Ru-jin,WU Kang-le,WANG Jian-min,MA Zhao-long. Resistant Mechanisms of Multi-drug Resistant Klebsiella pneumoniae[J]. Chinese Journal of Nosocomiology, 2010, 20(17) |
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| Authors: | ZHU Jian-ming SUN Xiao-zhen JIANG Ru-jin WU Kang-le WANG Jian-min MA Zhao-long |
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| Affiliation: | ZHU Jian-ming,SUN Xiao-zhen,JIANG Ru-jin,WU Kang-le,WANG Jian-min,MA Zhao-long(Hangzhou Yuhang Hospital of Traditional Chinese Medicine,Hangzhou,Zhejiang 311106,China) |
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| Abstract: | OBJECTIVE To investigate the distribution of 58 kinds of resistance genes and mobile genetic elements in a multi drug resistant Klebsiella pneumoniae(MDRKP) strain.METHODS This MDRKP strain was collected from a hospital in China.Then,58 kinds of genes resistant to beta-lactams,aminoglycosides,quinolones,and other broad-spectrum antimicrobial agents as well as mobile genetic elements were analyzed by PCR and verificated by DNA sequencing.RESULTS TEM-1,SHV-11(both were verificated by DNA sequencing),tetA,mdfA... |
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| Keywords: | Klebsiella pneumoniae Resistance gene Multi-drug resistance Trapping mechanism |
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