| 电化学检测器的研制及用以测定生物胺的初步实验 |
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| 引用本文： | 杨德隆,吴贵宝,李桂云,李玲. 电化学检测器的研制及用以测定生物胺的初步实验[J]. 华中科技大学学报(医学版), 1992, 0(5) |
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| 作者姓名： | 杨德隆 吴贵宝 李桂云 李玲 |
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| 作者单位： | 同济医科大学实验医学研究中心分析室(杨德隆，吴贵宝，李桂云)，同济医科大学实验医学研究中心分析室(李玲) |
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| 摘 要： | 研制一种用于高压液相色谱(HPLC)的电化学检测器(ECD)。用该检测器检测了从色谱柱分离出的4种生物胺:肾上腺素(EA)、去甲肾上腺索(NE)、多巴胺(DA)和5-羟色胺(5HT)。结果显示:在0.25～2.25ng范围内线性响应良好;峰高重复性相对偏差2.3％(对1.0ng NE)。EA、NE、DA和5-HT的检测限分别为0.06、0.06,0.07,0.10 ng(信噪比为2.5:1)。
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| 关 键 词： | 电化学检测器 色谱法 高压液相 生物胺类 |
Design, Manufacture and Preliminary Test of Electrochemical Detector for Detecting Biogenic Amines |
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| Yan Delong,Wu Guibao,Li Guiyun,Li LingLaboratory of Analysis,Research Center of ExperimentalMedicine,Tongji Medical University,Wuhan. Design, Manufacture and Preliminary Test of Electrochemical Detector for Detecting Biogenic Amines[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1992, 0(5) |
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| Authors: | Yan Delong Wu Guibao Li Guiyun Li LingLaboratory of Analysis Research Center of ExperimentalMedicine Tongji Medical University Wuhan |
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| Abstract: | An electrochemical detector for HPLC was designed and manufactured. The detector was utilized to detect four biogenic amines (EA, NE, DA, and 5-HT) separated on the strong cation resin column. The linear response was good in the range of 0.25 - 2.25 ng. The relative deviation of peak height reproducibility was 2..3% (for 1.0 ng NE). The detecting limit for EA, NE, DA, 5-HT was 0.06, 0.06, 0.07, and 0.1 ng respectively. |
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| Keywords: | electrochemical detector chromatography high pressure liquid biogenic amines |
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