金鸡菊提取物降压及体内抗氧化作用的研究

目的:研究金鸡菊提取物50％乙醇洗脱物对高血压小鼠的降压作用和体内抗氧化作用.方法:采用低温高盐法,连续20 d形成小鼠高血压模型；将实验小鼠分为空白组、模型组、金鸡菊组(100,200 mg· kg -1)、阳性药组(50 mg·kg-1).灌胃给药28 d后,采用颈动脉插管法测定小鼠血压并取血,检测血清中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量；称取肝脏,测定肝组织中SOD,MDA,谷胱甘肽过氧化物酶(GSH-Px)活力、总抗氧化能力(T-AOC).结果:与空白组相比,模型组小鼠血压明显降低,并具有显著性差异(P＜0.01)；血清和肝脏中的SOD,GSH-Px,T-AOC活力明显降低,MDA含量明显升高,统计结果显示有显著性差异(P＜0.01)；而与模型组比较,给药组高,低剂量可明显降低小鼠血压；同时,血清和肝脏中SOD,GSH-Px,T-AOC活力均显著提高,高剂量组MDA含量明显降低,统计结果显示有显著性差异(P＜0.01).结论:金鸡菊提取物50％乙醇洗脱物(100,200 mg· kg-1)具有显著的降压效果,并且能显著增强高血压小鼠的体内抗氧化能力,减少高血压造成的氧化损伤,保护血管和脏器的损伤,研究结果表明这可能是金鸡菊能有效降低血压的作用机制之一.

Experimental Study on Antihypertension and in vivo Antioxidant Function of Coreopsis Extract

Objective: To study the influence of Coreopsis extract eluted by 50% ethanol on antihypertension and in vivo antioxidant function of hypertension mice. Method: Hypertension model of mice were induced by giving high salinity water continuously by gastric irrigation(ig) and keeping them in low-temperature environment 4 hours a day for 20 days. Then the mice were given drug continuously by ig for 28 days, measured blood pressure,collected blood and liver, and detected the activities of SOD,MDA in serum; meanwhile the livers were sampled for measurement of MDA content,activities of SOD,GSH-Px and T-AOC. Result: Compared with blank group, the blood pressure of control group was depressed significantly; SOD,GSH-Px and T-AOC activities of control group both in serum and livers were reduced significantly, results above indicated that the model was set up successfully. The drug can depress blood pressure,increase SOD,GSH-Px and T-AOC activities, reduce the content of MDA compared with control group. Conclusion: Coreopsis extract can significantly depress blood pressure,increase the ability of antioxidant effect of hypertension mouse in vivo, and reduce oxidation injury caused by hypertension, protect blood vessel and internal organs had been injuried. All results indicated that it is one of the mechanisms of antihypertensive effects of Coreopsis extract.