| 华支睾吸虫感染小鼠肝脏中TLR2 mRNA动态表达的初步研究 |
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| 引用本文： | 徐田云,汤仁仙,刘转转,付琳琳,万青,刘宜升,孔德龙,尤红娟,杜文平,刘瀛,郑葵阳. 华支睾吸虫感染小鼠肝脏中TLR2 mRNA动态表达的初步研究[J]. 中国病原生物学杂志, 2011, 0(8) |
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| 作者姓名： | 徐田云 汤仁仙 刘转转 付琳琳 万青 刘宜升 孔德龙 尤红娟 杜文平 刘瀛 郑葵阳 |
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| 作者单位： | 徐州医学院病原生物与免疫学教研室; |
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| 基金项目： | 江苏省高校自然科学研究项目(No.07KJB310122); 江苏省研究生培养创新工程项目(No.CX07S-038z) |
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| 摘 要： | 目的观察华支睾吸虫感染小鼠肝脏中TLR2 mRNA的动态表达。方法建立华支睾吸虫感染小鼠模型,在感染不同时间点(1、2、4、7、14、28、568、4 d)取肝脏,采用逆转录聚合酶链反应检测肝脏TLR2 mRNA的表达,同时行HE染色观察感染小鼠各时间点肝脏病理学变化。结果与未感染对照组相比,感染组自感染后第7 d起TLR2 mRNA表达升高(P<0.05),至第28 d达到高峰,之后开始下降,至第56 d仍高于未感染对照组(P<0.05),第84 d下降至未感染对照组相近水平。病理学观察发现,感染小鼠肝脏于第4 d始,汇管区轻度炎症反应;第7 d,炎症反应加重,或见嗜酸性脓肿;第14 d,嗜酸性脓肿增多,纤维组织增生,周围小胆管增生;第28 d,胆管壁增厚,周围炎性渗出明显;第56 d,胆管上皮不同程度增生,增生的上皮呈腺样结构;第84 d,胆管周围炎性细胞减少,纤维增多,管壁增厚。结论 TLR2 mRNA在华支睾吸虫感染过程中表达升高,推测TLR2可能参与调控华支睾吸虫感染引发的Th1/Th2免疫应答和病理改变。
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| 关 键 词： | Toll样受体 华支睾吸虫 小鼠 肝脏 |
An initial study of dynamic expression of TLR2 mRNA in the livers of mice infected with Clonorchis sinensis |
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| XU Tian-yun,TANG Ren-xian,LIU Zhuan-zhuan,FU Lin-lin,WAN Qing,LIU Yi-sheng,KONG De-long,YOU Hong-juan,DU Wen-ping,LIU Ying,ZHENG Kui-yang. An initial study of dynamic expression of TLR2 mRNA in the livers of mice infected with Clonorchis sinensis[J]. Journal of Pathogen Biology, 2011, 0(8) |
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| Authors: | XU Tian-yun TANG Ren-xian LIU Zhuan-zhuan FU Lin-lin WAN Qing LIU Yi-sheng KONG De-long YOU Hong-juan DU Wen-ping LIU Ying ZHENG Kui-yang |
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| Affiliation: | XU Tian-yun,TANG Ren-xian,LIU Zhuan-zhuan,FU Lin-lin,WAN Qing,LIU Yi-sheng,KONG De-long,YOU Hong-juan,DU Wen-ping,LIU Ying,ZHENG Kui-yang(Department of Pathogenic Biology and Immunology,Xuzhou Medical College,Xuzhou 221002,Jiangsu,China) |
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| Abstract: | Objective The dynamic expression of TLR2 mRNA in liver of mice infected with Clonorchis sinensis was investigated to provide a basis for the role of TLR2 in the pathogenesis of clonorchiasis sinensis.Methods A well characterized mouse model of clonorchiasis sinensis was used in this study.Livers were removed from mice at various times post-infection(1 d,2 d,4 d,7 d,14 d,28 d,56 d,and 84 d).The dynamic expression of TLR2 mRNA in livers was detected with RT-PCR.Pathological changes in the liver were observed ... |
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| Keywords: | Toll-like receptor(TLR) Clonorchis sinensis mouse liver |
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